Phyllanthus amarus down-regulates hepatitis B virus mRNA transcription and replication

C. D. Lee, M. Ott, S. P. Thyagarajan, D. A. Shafritz, R. D. Burk, S. Gupta

Research output: Contribution to journalArticle

95 Scopus citations

Abstract

The Phyllanthus amarus plant shows potential for treating hepatitis B virus. To define the mechanism of action of P. amarus, we used HepG2 2.2.15 cells, which support hepatitis B virus replication. P. amarus inhibited hepatitis B virus polymerase activity, decreased episomal hepatitis B virus DNA content and suppressed virus release into culture medium. To examine transcriptional control mechanisms, we used G26 hepatitis B virus transgenic mice, which produce serum HBsAg but neither HBcAg nor virion particles. When P. amarus was administered to transgenic mice, hepatic HBsAg mRNA levels decreased, indicating transcriptional or post-transcriptional down-regulation of the transgene. Increase in hepatitis B virus mRNA expression after stimulation of the glucocorticoid responsive element was also suppressed by P. amarus, suggesting involvement of the hepatitis B virus enhancer in this response. Disruption by P. amarus of hepatitis B virus polymerase activity, mRNA transcription and replication supports its role as an antiviral agent.

Original languageEnglish (US)
Pages (from-to)1069-1076
Number of pages8
JournalEuropean Journal of Clinical Investigation
Volume26
Issue number12
DOIs
StatePublished - 1996

Keywords

  • Gene expression
  • Hepatitis B virus
  • Liver
  • Medicinal plants
  • Transgenic mouse

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

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