Photographic and luminometric detection of luciferase reporter phages for drug susceptibility testing of clinical Mycobacterium tuberculosis isolates

Manzour Hernando Hazbón, Nora Guarín, Beatriz Eugenia Ferro, Ana Lucía Rodríguez, Luz Angela Labrada, Rafael Tovar, Paul F. Riska, William R. Jacobs

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Luciferase reporter phages (LRPs) have proven to be efficient tools for drug. susceptibility testing of Mycobacterium tuberculosis. Luminometric detection of LRP activity offers higher sensitivity and quantitative results, while a Polaroid film detection method offers a "low-tech" inexpensive alternative that is called the Bronx box. In this work we evaluated, improved, and compared the performance of the luminometer and the Bronx box formats for drug susceptibility testing with LRPs by using 51 clinical isolates of M. tuberculosis, with the agar proportion method (PM) serving as reference. The sensitivity in detecting resistance to isoniazid and rifampin, antibiotics that define multidrug resistance (MDR), was 100% for both methods. The turnaround time for results was reduced from 3 weeks for PM to 54 or 94 h for luminometry or the Bronx box, respectively. These results support the utility of LRPs as a screening test for the surveillance of MDR tuberculosis.

Original languageEnglish (US)
Pages (from-to)4865-4869
Number of pages5
JournalJournal of Clinical Microbiology
Volume41
Issue number10
DOIs
StatePublished - Oct 1 2003
Externally publishedYes

Fingerprint

Luciferases
Mycobacterium tuberculosis
Bacteriophages
Multiple Drug Resistance
Pharmaceutical Preparations
Isoniazid
Rifampin
Agar
Tuberculosis
Anti-Bacterial Agents

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Photographic and luminometric detection of luciferase reporter phages for drug susceptibility testing of clinical Mycobacterium tuberculosis isolates. / Hazbón, Manzour Hernando; Guarín, Nora; Ferro, Beatriz Eugenia; Rodríguez, Ana Lucía; Labrada, Luz Angela; Tovar, Rafael; Riska, Paul F.; Jacobs, William R.

In: Journal of Clinical Microbiology, Vol. 41, No. 10, 01.10.2003, p. 4865-4869.

Research output: Contribution to journalArticle

Hazbón, Manzour Hernando ; Guarín, Nora ; Ferro, Beatriz Eugenia ; Rodríguez, Ana Lucía ; Labrada, Luz Angela ; Tovar, Rafael ; Riska, Paul F. ; Jacobs, William R. / Photographic and luminometric detection of luciferase reporter phages for drug susceptibility testing of clinical Mycobacterium tuberculosis isolates. In: Journal of Clinical Microbiology. 2003 ; Vol. 41, No. 10. pp. 4865-4869.
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