Photocontrollable fluorescent proteins for superresolution imaging

Daria Shcherbakova, Prabuddha Sengupta, Jennifer Lippincott-Schwartz, Vladislav Verkhusha

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

Superresolution fluorescence microscopy permits the study of biological processes at scales small enough to visualize fine subcellular structures that are unresolvable by traditional diffraction-limited light microscopy. Many superresolution techniques, including those applicable to live cell imaging, utilize genetically encoded photocontrollable fluorescent proteins. The fluorescence of these proteins can be controlled by light of specific wavelengths. In this review, we discuss the biochemical and photophysical properties of photocontrollable fluorescent proteins that are relevant to their use in superresolution microscopy. We then describe the recently developed photoactivatable, photoswitchable, and reversibly photoswitchable fluorescent proteins, and we detail their particular usefulness in single-molecule localization-based and nonlinear ensemble-based superresolution techniques. Finally, we discuss recent applications of photocontrollable proteins in superresolution imaging, as well as how these applications help to clarify properties of intracellular structures and processes that are relevant to cell and developmental biology, neuroscience, cancer biology and biomedicine.

Original languageEnglish (US)
Pages (from-to)303-329
Number of pages27
JournalAnnual Review of Biophysics
Volume43
Issue number1
DOIs
StatePublished - 2014

Fingerprint

Proteins
Imaging techniques
Microscopy
Cytology
Biological Phenomena
Light
Developmental Biology
Fluorescence microscopy
Neurosciences
Fluorescence Microscopy
Optical microscopy
Cell Biology
Microscopic examination
Diffraction
Fluorescence
Wavelength
Molecules
Neoplasms

Keywords

  • EosFP
  • PAGFP
  • PALM
  • PAmCherry
  • RESOLFT

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Structural Biology
  • Bioengineering

Cite this

Photocontrollable fluorescent proteins for superresolution imaging. / Shcherbakova, Daria; Sengupta, Prabuddha; Lippincott-Schwartz, Jennifer; Verkhusha, Vladislav.

In: Annual Review of Biophysics, Vol. 43, No. 1, 2014, p. 303-329.

Research output: Contribution to journalArticle

Shcherbakova, Daria ; Sengupta, Prabuddha ; Lippincott-Schwartz, Jennifer ; Verkhusha, Vladislav. / Photocontrollable fluorescent proteins for superresolution imaging. In: Annual Review of Biophysics. 2014 ; Vol. 43, No. 1. pp. 303-329.
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