Phosphorylation of human small heat shock protein HspB8 (Hsp22) by ERK1 protein kinase

Anton Shemetov, Alim S. Seit-Nebi, Nikolai B. Gusev

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A number of phosphomimicking mutants (replacement of Ser/Thr residues by Asp) of human small heat shock protein HspB8 were obtained and phosphorylation of the wild type HspB8 and its mutants by ERK1 kinase was analyzed in vitro. Mutation S159D does not affect phosphorylation, whereas mutations S24D and S27D equally moderately inhibited and mutation T87D strongly inhibited phosphorylation of HspB8. The double mutations S24D/T87D and S27D/T87D induced very strong inhibitory effect and the triple mutations S24D/S27D/T87D completely prevented phosphorylation catalyzed by ERK1. Thus, Ser24 and Thr87, found to be phosphorylated in vivo, are among the sites phosphorylated by ERK1 in HspB8 in vitro. Mutations S24D and T87D affect intrinsic tryptophan fluorescence and susceptibility to chymotrypsinolysis of HspB8. Phosphomimicking mutations and phosphorylation promote concentration-dependent association of HspB8 subunits. Mutations S24D and S27D decrease, whereas mutation T87D increases the chaperone-like activity of HspB8. It is concluded that phosphorylation catalyzed by ERK1 might affect the structure and chaperone-like activity of HspB8 and therefore can be important for regulation of interaction of HspB8 with different target proteins.

Original languageEnglish (US)
Pages (from-to)47-55
Number of pages9
JournalMolecular and Cellular Biochemistry
Volume355
Issue number1-2
DOIs
StatePublished - Sep 1 2011
Externally publishedYes

Fingerprint

Small Heat-Shock Proteins
Phosphorylation
Mitogen-Activated Protein Kinase 3
Protein Kinases
Mutation
Tryptophan
Fluorescence
Association reactions
Viperidae

Keywords

  • Chaperone-like activity
  • Human small heat shock protein HspB8
  • Phosphorylation
  • Structure

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Phosphorylation of human small heat shock protein HspB8 (Hsp22) by ERK1 protein kinase. / Shemetov, Anton; Seit-Nebi, Alim S.; Gusev, Nikolai B.

In: Molecular and Cellular Biochemistry, Vol. 355, No. 1-2, 01.09.2011, p. 47-55.

Research output: Contribution to journalArticle

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