Phosphorus-31 relaxation rate studies of Mn2+ - alkaline phosphatase

R. Suzanne Zukin, Donald P. Hollis, George A. Gray

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Phosphorus-31 NMR relaxation rates for the ternary complex of manganese-alkaline phosphatase-phosphate have been measured and their temperature dependence studied. The exchange of phosphate into the complex is exchange limited with respect to the transverse relaxation rate but is fast with respect to longitudinal relaxation. The data show that the observed phosphate relaxation is an outer-sphere effect. The activation energy for phosphate exchange is Ea = 8 Kcal/mole as determined from the temperature dependence of the line width of the phosphorus resonance.

Original languageEnglish (US)
Pages (from-to)238-243
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume53
Issue number1
DOIs
StatePublished - Jul 2 1973
Externally publishedYes

Fingerprint

Phosphorus
Alkaline Phosphatase
Phosphates
Temperature
Manganese
Linewidth
Activation energy
Nuclear magnetic resonance

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Phosphorus-31 relaxation rate studies of Mn2+ - alkaline phosphatase. / Zukin, R. Suzanne; Hollis, Donald P.; Gray, George A.

In: Biochemical and Biophysical Research Communications, Vol. 53, No. 1, 02.07.1973, p. 238-243.

Research output: Contribution to journalArticle

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