Phosphorus-31 relaxation rate studies of Mn2+ - alkaline phosphatase

Ruth S. Zukin; Donald P. Hollis; George A. Gray

doi:10.1016/0006-291X(73)91425-3

Phosphorus-31 relaxation rate studies of Mn²⁺ - alkaline phosphatase

Ruth S. Zukin, Donald P. Hollis, George A. Gray

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Phosphorus-31 NMR relaxation rates for the ternary complex of manganese-alkaline phosphatase-phosphate have been measured and their temperature dependence studied. The exchange of phosphate into the complex is exchange limited with respect to the transverse relaxation rate but is fast with respect to longitudinal relaxation. The data show that the observed phosphate relaxation is an outer-sphere effect. The activation energy for phosphate exchange is E_a = 8 Kcal/mole as determined from the temperature dependence of the line width of the phosphorus resonance.

Original language	English (US)
Pages (from-to)	238-243
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	53
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(73)91425-3
State	Published - Jul 2 1973
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/0006-291X(73)91425-3

Cite this

@article{786afa361fcc4eb6942338074014b746,

title = "Phosphorus-31 relaxation rate studies of Mn2+ - alkaline phosphatase",

abstract = "Phosphorus-31 NMR relaxation rates for the ternary complex of manganese-alkaline phosphatase-phosphate have been measured and their temperature dependence studied. The exchange of phosphate into the complex is exchange limited with respect to the transverse relaxation rate but is fast with respect to longitudinal relaxation. The data show that the observed phosphate relaxation is an outer-sphere effect. The activation energy for phosphate exchange is Ea = 8 Kcal/mole as determined from the temperature dependence of the line width of the phosphorus resonance.",

author = "Zukin, {Ruth S.} and Hollis, {Donald P.} and Gray, {George A.}",

year = "1973",

month = jul,

day = "2",

doi = "10.1016/0006-291X(73)91425-3",

language = "English (US)",

volume = "53",

pages = "238--243",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Phosphorus-31 relaxation rate studies of Mn2+ - alkaline phosphatase

AU - Zukin, Ruth S.

AU - Hollis, Donald P.

AU - Gray, George A.

PY - 1973/7/2

Y1 - 1973/7/2

N2 - Phosphorus-31 NMR relaxation rates for the ternary complex of manganese-alkaline phosphatase-phosphate have been measured and their temperature dependence studied. The exchange of phosphate into the complex is exchange limited with respect to the transverse relaxation rate but is fast with respect to longitudinal relaxation. The data show that the observed phosphate relaxation is an outer-sphere effect. The activation energy for phosphate exchange is Ea = 8 Kcal/mole as determined from the temperature dependence of the line width of the phosphorus resonance.

AB - Phosphorus-31 NMR relaxation rates for the ternary complex of manganese-alkaline phosphatase-phosphate have been measured and their temperature dependence studied. The exchange of phosphate into the complex is exchange limited with respect to the transverse relaxation rate but is fast with respect to longitudinal relaxation. The data show that the observed phosphate relaxation is an outer-sphere effect. The activation energy for phosphate exchange is Ea = 8 Kcal/mole as determined from the temperature dependence of the line width of the phosphorus resonance.

UR - http://www.scopus.com/inward/record.url?scp=0015912486&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015912486&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(73)91425-3

DO - 10.1016/0006-291X(73)91425-3

M3 - Article

C2 - 4582371

AN - SCOPUS:0015912486

SN - 0006-291X

VL - 53

SP - 238

EP - 243

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Phosphorus-31 relaxation rate studies of Mn²⁺ - alkaline phosphatase

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this