Phosphatidylinositol 4,5-bisphosphate regulates adipocyte actin dynamics and GLUT4 vesicle recycling

Makoto Kanzaki, Megumi Furukawa, William Raab, Jeffrey E. Pessin

Research output: Contribution to journalArticle

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Abstract

To investigate the potential role of phosphatidylinositol 4, 5-bisphosphate (PI(4,5)P2) in the regulation of actin polymerization and GLUT4 translocation, the type I phosphatidylinositol 4-phosphate 5-kinases (PIP5Ks) were expressed in 3T3L1 adipocytes. In preadipocytes (fibroblasts) PIP5K expression promoted actin polymerization on membrane-bound vesicles to form motile actin comets. In contrast, expression of PIP5K in differentiated 3T3L1 adipocytes resulted in the formation of enlarged vacuole-like structures coated with F-actin, cortactin, dynamin, and N-WASP. Treatment with either latrunculin B (an inhibitor for actin polymerization) or Clostridium difficile toxin B (a general Rho family inhibitor) resulted in a relatively slower disappearance of coated F-actin from these vacuoles, but the vacuoles themselves remained unaffected. Functionally, the increased PI(4,5)P2 levels resulted in an inhibition of transferrin receptor and GLUT4 endocytosis and a slow accumulation of these proteins in the PI(4,5)P2-enriched vacuoles along with the non-clathrin-derived endosome marker (caveolin) and the AP-2 adaptor complex. However, these structures were devoid of early endosome markers (EEA1, clathrin) and the biosynthetic membrane secretory machinery markers p115 (Golgi) and syntaxin 6 (trans-Golgi Network). Taken together, these data demonstrate that PI(4,5)P2 has distinct morphologic and functional properties depending upon specific cell context. In adipocytes, altered PI(4,5)P2 metabolism has marked effects on GLUT4 endocytosis and intracellular vesicle trafficking due to the derangement of actin dynamics.

Original languageEnglish (US)
Pages (from-to)30622-30633
Number of pages12
JournalJournal of Biological Chemistry
Volume279
Issue number29
DOIs
StatePublished - Jul 16 2004
Externally publishedYes

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Recycling
Phosphatidylinositols
Adipocytes
Actins
Vacuoles
Polymerization
Endosomes
Endocytosis
Caveolin 2
Cortactin
Qa-SNARE Proteins
Caveolins
Dynamins
Membranes
trans-Golgi Network
Clathrin
Transferrin Receptors
Fibroblasts
Metabolism
Machinery

ASJC Scopus subject areas

  • Biochemistry

Cite this

Phosphatidylinositol 4,5-bisphosphate regulates adipocyte actin dynamics and GLUT4 vesicle recycling. / Kanzaki, Makoto; Furukawa, Megumi; Raab, William; Pessin, Jeffrey E.

In: Journal of Biological Chemistry, Vol. 279, No. 29, 16.07.2004, p. 30622-30633.

Research output: Contribution to journalArticle

Kanzaki, Makoto ; Furukawa, Megumi ; Raab, William ; Pessin, Jeffrey E. / Phosphatidylinositol 4,5-bisphosphate regulates adipocyte actin dynamics and GLUT4 vesicle recycling. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 29. pp. 30622-30633.
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