Phorbol myristic acetate markedly stimulated LAF production by the murine macrophage cell line, P388D1. This effect was both time and concentration dependent. Other analogs, such as phorbol didecanoate and to a lesser extent, phorbol dibenzoate also enhanced LAF production, but the parent compound, phorbol, was inactive. The PMA induced supernatant LAF had a molecular weight of 16,000 daltons and exhibited charge heterogenity on DEAE cellulose. Since PMA is a small compound of defined structure which induces the production of large amounts of LAF it should be a useful tool with which to probe the biology and biochemistry of LAF.
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