Phorbol myristic acetate stimulates LAF production by the macrophage cell line, P388D

Steven B. Mizel; David L. Rosenstreich; Joost J. Oppenheim

doi:10.1016/0008-8749(78)90330-1

Phorbol myristic acetate stimulates LAF production by the macrophage cell line, P388D

Steven B. Mizel, David L. Rosenstreich, Joost J. Oppenheim

Research output: Contribution to journal › Article › peer-review

101 Scopus citations

Abstract

Phorbol myristic acetate markedly stimulated LAF production by the murine macrophage cell line, P388D₁. This effect was both time and concentration dependent. Other analogs, such as phorbol didecanoate and to a lesser extent, phorbol dibenzoate also enhanced LAF production, but the parent compound, phorbol, was inactive. The PMA induced supernatant LAF had a molecular weight of 16,000 daltons and exhibited charge heterogenity on DEAE cellulose. Since PMA is a small compound of defined structure which induces the production of large amounts of LAF it should be a useful tool with which to probe the biology and biochemistry of LAF.

Original language	English (US)
Pages (from-to)	230-235
Number of pages	6
Journal	Cellular Immunology
Volume	40
Issue number	1
DOIs	https://doi.org/10.1016/0008-8749(78)90330-1
State	Published - Sep 15 1978
Externally published	Yes

ASJC Scopus subject areas

Immunology

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title = "Phorbol myristic acetate stimulates LAF production by the macrophage cell line, P388D",

abstract = "Phorbol myristic acetate markedly stimulated LAF production by the murine macrophage cell line, P388D1. This effect was both time and concentration dependent. Other analogs, such as phorbol didecanoate and to a lesser extent, phorbol dibenzoate also enhanced LAF production, but the parent compound, phorbol, was inactive. The PMA induced supernatant LAF had a molecular weight of 16,000 daltons and exhibited charge heterogenity on DEAE cellulose. Since PMA is a small compound of defined structure which induces the production of large amounts of LAF it should be a useful tool with which to probe the biology and biochemistry of LAF.",

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T1 - Phorbol myristic acetate stimulates LAF production by the macrophage cell line, P388D

AU - Mizel, Steven B.

AU - Rosenstreich, David L.

AU - Oppenheim, Joost J.

PY - 1978/9/15

Y1 - 1978/9/15

N2 - Phorbol myristic acetate markedly stimulated LAF production by the murine macrophage cell line, P388D1. This effect was both time and concentration dependent. Other analogs, such as phorbol didecanoate and to a lesser extent, phorbol dibenzoate also enhanced LAF production, but the parent compound, phorbol, was inactive. The PMA induced supernatant LAF had a molecular weight of 16,000 daltons and exhibited charge heterogenity on DEAE cellulose. Since PMA is a small compound of defined structure which induces the production of large amounts of LAF it should be a useful tool with which to probe the biology and biochemistry of LAF.

AB - Phorbol myristic acetate markedly stimulated LAF production by the murine macrophage cell line, P388D1. This effect was both time and concentration dependent. Other analogs, such as phorbol didecanoate and to a lesser extent, phorbol dibenzoate also enhanced LAF production, but the parent compound, phorbol, was inactive. The PMA induced supernatant LAF had a molecular weight of 16,000 daltons and exhibited charge heterogenity on DEAE cellulose. Since PMA is a small compound of defined structure which induces the production of large amounts of LAF it should be a useful tool with which to probe the biology and biochemistry of LAF.

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Phorbol myristic acetate stimulates LAF production by the macrophage cell line, P388D

Abstract

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