Phorbol myristic acetate stimulates LAF production by the macrophage cell line, P388D

Steven B. Mizel, David L. Rosenstreich, Joost J. Oppenheim

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Abstract

Phorbol myristic acetate markedly stimulated LAF production by the murine macrophage cell line, P388D1. This effect was both time and concentration dependent. Other analogs, such as phorbol didecanoate and to a lesser extent, phorbol dibenzoate also enhanced LAF production, but the parent compound, phorbol, was inactive. The PMA induced supernatant LAF had a molecular weight of 16,000 daltons and exhibited charge heterogenity on DEAE cellulose. Since PMA is a small compound of defined structure which induces the production of large amounts of LAF it should be a useful tool with which to probe the biology and biochemistry of LAF.

Original languageEnglish (US)
Pages (from-to)230-235
Number of pages6
JournalCellular Immunology
Volume40
Issue number1
DOIs
Publication statusPublished - Sep 15 1978
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology

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