Phenotypic characterization of white cells in white cell-reduced red cell concentrate using flow cytometry.

Edward R. Burns, E. R. Burns

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The residual white cell (WBC) content of donated units of red cell concentrate rendered WBC-reduced by filtration through commercially available polyester filters was quantified and phenotypically analyzed. All studies were performed by flow cytometery. Quantification studies were performed with a DNA/RNA fluorophore, propidium iodide. WBC subset analyses were performed with fluorescence-labeled monoclonal antibodies directed against various cluster differentiation (CD) loci. The results indicate that the filter removes in excess of 3 log10 total WBCs from the red cell components and depletes granulocytes to or beyond the specific assay's sensitivity of 3 log10. Total T and B cells, T4 and T8 lymphocytes, and monocytes are reduced by approximately 4 log10. These analyses provide plausible explanations for the clinical success of the filter and suggest other potential applications.

Original languageEnglish (US)
Pages (from-to)829-834
Number of pages6
JournalTransfusion
Volume31
Issue number9
StatePublished - Nov 1991

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Flow Cytometry
CD8-Positive T-Lymphocytes
CD4-Positive T-Lymphocytes
Polyesters
Propidium
Cellular Structures
Granulocytes
Monocytes
B-Lymphocytes
Fluorescence
Monoclonal Antibodies
RNA
T-Lymphocytes
DNA

ASJC Scopus subject areas

  • Immunology
  • Hematology

Cite this

Phenotypic characterization of white cells in white cell-reduced red cell concentrate using flow cytometry. / Burns, Edward R.; Burns, E. R.

In: Transfusion, Vol. 31, No. 9, 11.1991, p. 829-834.

Research output: Contribution to journalArticle

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