Phenothiazines inhibit S100A4 function by inducing protein oligomerization

Vladimir N. Malashkevich, Natalia Dulianinova, Udupi A. Ramagopal, Melissa A. Liriano, Kristen M. Varney, David Knight, Michael D. Brenowitz, David J. Weber, Steven C. Almo, Anne R. Bresnick

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

S100A4, a member of the S100 family of Ca22+-binding proteins, regulates carcinoma cell motility via interactions with myosin-IIA. Numerous studies indicate that S100A4 is not simply a marker for metastatic disease, but rather has a direct role in metastatic progression. These observations suggest that S100A4 is an excellent target for therapeutic intervention. Using a unique biosensor-based assay, trifluoperazine (TFP) was identified as an inhibitor that disrupts the S100A4/myosin-IIA interaction. To examine the interaction of S100A4 with TFP, we determined the 2.3 Å crystal structure of human Ca2+-S100A4 bound to TFP. Two TFP molecules bind within the hydrophobic target binding pocket of Ca2+-S100A4 with no significant conformational changes observed in the protein upon complex formation. NMR chemical shift perturbations are consistent with the crystal structure and demonstrate that TFP binds to the target binding cleft of S100A4 in solution. Remarkably, TFP binding results in the assembly of five Ca2+-S100A4/ TFP dimers into a tightly packed pentameric ring. Within each pentamer most of the contacts between S100A4 dimers occurs through the TFP moieties. The Ca 2+-S100A4/prochlorperazine (PCP) complex exhibits a similar pentameric assembly. Equilibrium sedimentation and cross-linking studies demonstrate the cooperative formation of a similarly sized S100A4/TFP oligomer in solution. Assays examining the ability of TFP to block S100A4-mediated disassembly of myosin-IIA filaments demonstrate that significant inhibition of S100A4 function occurs only at TFP concentrations that promote S100A4 oligomerization. Together these studies support a unique mode of inhibition in which phenothiazines disrupt the S100A4/ myosin-IIA interaction by sequestering S100A4 via small molecule-induced oligomerization.

Original languageEnglish (US)
Pages (from-to)8605-8610
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number19
DOIs
StatePublished - May 11 2010

Fingerprint

Phenothiazines
Trifluoperazine
Nonmuscle Myosin Type IIA
Proteins
Prochlorperazine
Aptitude
Biosensing Techniques
Cell Movement
Carrier Proteins

Keywords

  • Calcium
  • Metastasis
  • NMR
  • Small molecule inhibitor
  • X-ray crystallography

ASJC Scopus subject areas

  • General

Cite this

Phenothiazines inhibit S100A4 function by inducing protein oligomerization. / Malashkevich, Vladimir N.; Dulianinova, Natalia; Ramagopal, Udupi A.; Liriano, Melissa A.; Varney, Kristen M.; Knight, David; Brenowitz, Michael D.; Weber, David J.; Almo, Steven C.; Bresnick, Anne R.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, No. 19, 11.05.2010, p. 8605-8610.

Research output: Contribution to journalArticle

Malashkevich, Vladimir N. ; Dulianinova, Natalia ; Ramagopal, Udupi A. ; Liriano, Melissa A. ; Varney, Kristen M. ; Knight, David ; Brenowitz, Michael D. ; Weber, David J. ; Almo, Steven C. ; Bresnick, Anne R. / Phenothiazines inhibit S100A4 function by inducing protein oligomerization. In: Proceedings of the National Academy of Sciences of the United States of America. 2010 ; Vol. 107, No. 19. pp. 8605-8610.
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abstract = "S100A4, a member of the S100 family of Ca22+-binding proteins, regulates carcinoma cell motility via interactions with myosin-IIA. Numerous studies indicate that S100A4 is not simply a marker for metastatic disease, but rather has a direct role in metastatic progression. These observations suggest that S100A4 is an excellent target for therapeutic intervention. Using a unique biosensor-based assay, trifluoperazine (TFP) was identified as an inhibitor that disrupts the S100A4/myosin-IIA interaction. To examine the interaction of S100A4 with TFP, we determined the 2.3 {\AA} crystal structure of human Ca2+-S100A4 bound to TFP. Two TFP molecules bind within the hydrophobic target binding pocket of Ca2+-S100A4 with no significant conformational changes observed in the protein upon complex formation. NMR chemical shift perturbations are consistent with the crystal structure and demonstrate that TFP binds to the target binding cleft of S100A4 in solution. Remarkably, TFP binding results in the assembly of five Ca2+-S100A4/ TFP dimers into a tightly packed pentameric ring. Within each pentamer most of the contacts between S100A4 dimers occurs through the TFP moieties. The Ca 2+-S100A4/prochlorperazine (PCP) complex exhibits a similar pentameric assembly. Equilibrium sedimentation and cross-linking studies demonstrate the cooperative formation of a similarly sized S100A4/TFP oligomer in solution. Assays examining the ability of TFP to block S100A4-mediated disassembly of myosin-IIA filaments demonstrate that significant inhibition of S100A4 function occurs only at TFP concentrations that promote S100A4 oligomerization. Together these studies support a unique mode of inhibition in which phenothiazines disrupt the S100A4/ myosin-IIA interaction by sequestering S100A4 via small molecule-induced oligomerization.",
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