Phakomatous choristoma of the eyelid: Immunohistochemical and electron microscopic observations

P. S. Rosenbaum, Y. Kress, Thomas L. Slamovits, R. L. Font

Research output: Contribution to journalArticle

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Abstract

Background: A 13-month-old Hispanic boy underwent excision of a congenital inferonasal orbital mass arising from the right lower lid. Results of histopathologic examination of the tumor showed a phakomatous choristoma of the eyelid. An immunohistochemical and electron microscopic study of this rare, benign, congenital tumor of lenticular anlage was performed. Methods: Immunohistochemistry was performed on 4-μm thick sections from paraffin- embedded tissue. Electron microscopy was performed on thin sections stained with uranyl acetate and lead citrate. Findings: The cuboidal epithelial cells that comprise this choristoma showed strongly positive cytoplasmic staining with S-100 protein and vimentin and focally positive staining with a keratin cocktail (AE1/AE3). Electron microscopy showed the presence of numerous 10- nm whorled cytoplasmic microfilaments within degenerating epithelial cells. Conclusion: The immunoreactivity of this tumor to keratin and vimentin are newly described in this detailed clinicopathologic report and, together with its S-100 positivity, support the proposal that this tumor is of lenticular anlage. The authors hypothesize that the intracytoplasmic 10-nm intermediate filaments observed with electron microscopic examination within the epithelial cells that comprise this choristoma represent vimentin as detected by immunohistochemistry.

Original languageEnglish (US)
Pages (from-to)1779-1784
Number of pages6
JournalOphthalmology
Volume99
Issue number12
StatePublished - 1992

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Choristoma
Eyelids
Vimentin
Electrons
Epithelial Cells
Keratins
Neoplasms
Electron Microscopy
Immunohistochemistry
Staining and Labeling
Intermediate Filaments
S100 Proteins
Actin Cytoskeleton
Hispanic Americans
Citric Acid
Paraffin

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Phakomatous choristoma of the eyelid : Immunohistochemical and electron microscopic observations. / Rosenbaum, P. S.; Kress, Y.; Slamovits, Thomas L.; Font, R. L.

In: Ophthalmology, Vol. 99, No. 12, 1992, p. 1779-1784.

Research output: Contribution to journalArticle

Rosenbaum, P. S. ; Kress, Y. ; Slamovits, Thomas L. ; Font, R. L. / Phakomatous choristoma of the eyelid : Immunohistochemical and electron microscopic observations. In: Ophthalmology. 1992 ; Vol. 99, No. 12. pp. 1779-1784.
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AB - Background: A 13-month-old Hispanic boy underwent excision of a congenital inferonasal orbital mass arising from the right lower lid. Results of histopathologic examination of the tumor showed a phakomatous choristoma of the eyelid. An immunohistochemical and electron microscopic study of this rare, benign, congenital tumor of lenticular anlage was performed. Methods: Immunohistochemistry was performed on 4-μm thick sections from paraffin- embedded tissue. Electron microscopy was performed on thin sections stained with uranyl acetate and lead citrate. Findings: The cuboidal epithelial cells that comprise this choristoma showed strongly positive cytoplasmic staining with S-100 protein and vimentin and focally positive staining with a keratin cocktail (AE1/AE3). Electron microscopy showed the presence of numerous 10- nm whorled cytoplasmic microfilaments within degenerating epithelial cells. Conclusion: The immunoreactivity of this tumor to keratin and vimentin are newly described in this detailed clinicopathologic report and, together with its S-100 positivity, support the proposal that this tumor is of lenticular anlage. The authors hypothesize that the intracytoplasmic 10-nm intermediate filaments observed with electron microscopic examination within the epithelial cells that comprise this choristoma represent vimentin as detected by immunohistochemistry.

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