Abstract
pH-induced closure of connexin43 (Cx43) channels involves interaction of the Cx43 carboxyl-terminal (Cx43CT) with a separate "receptor" domain. The receptor location and structure and whether the interaction is directly intramolecular are unknown. Here we show resonant mirror technology, enzyme-linked sorbent assays, and nuclear magnetic resonance (NMR) experiments demonstrating pH-dependent binding of Cx43CT to region 119-144 of Cx43 (Cx43L2), which we propose is the receptor. NMR showed that acidification induced α-helical order in Cx43L2, whereas only a minor modification in Cx43CT structure was detected. These data provide the first demonstration of chemically induced structural order and binding between cytoplasmic connexin domains.
Original language | English (US) |
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Pages (from-to) | 36706-36714 |
Number of pages | 9 |
Journal | Journal of Biological Chemistry |
Volume | 277 |
Issue number | 39 |
DOIs | |
State | Published - Sep 27 2002 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology