Persistent productive HIV-1 infection of a CD4- human fetal thymocyte line

William C. Hatch, Kathryn E. Tanaka, Theresa Calvelli, William K. Rashbaum, Yvonne Kress, William D. Lyman

Research output: Contribution to journalArticle

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Abstract

Human fetal thymuses were obtained from abortuses of HIV-1 seronegative females. Thymocytes were isolated and cultured for 2 days with PHA. Thereafter, the culture was divided and half of the cells were exposed to the HIV-1 RF isolate for 4 h. After this incubation period, the HIV-1 exposed and nonexposed control cells were cultured in RPMI 1640 supplemented with IL-2 for 30 days and subsequently maintained in RPMI without the addition of growth factors. Long term culture of both HIV-1 exposed and control thymocytes has yielded two cell lines that have been maintained for more than 3 yr without the addition of growth factors. Flow cytometry using mAb that recognize T cell differentiation markers was used to analyze cell phenotypes. The HIV-1 exposed thymocyte cell line (E88/ RF) was shown to be HIV-1 infected by p24 ELISA, reverse transcriptase activity, immunocytochemistry, in situ hybridization, polymerase chain reaction, electron microscopy, and to produce infectious particles by a syncytial forming assay. The non-HIV-1-exposed thymocyte cell line (T412) has remained negative by all criteria for HIV-1 infection. Flow cytometry showed the T412 cells to be positive for the T cell markers CD45, CD38, and CD4 but negative for all other markers tested. The E88/RF cells are positive for CD45 and CD38 but negative for CD4 and other markers. These data report the isolation of two human fetal thymocyte cell lines; one uninfected and susceptible to HIV-1 infection, and the other persistently and productively infected with HIV-1 with little cytopathology. These findings suggest that HIV-1 can persistently infect early T cells and may alter T cell differentiation.

Original languageEnglish (US)
Pages (from-to)3055-3061
Number of pages7
JournalJournal of Immunology
Volume148
Issue number10
StatePublished - May 15 1992

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Thymocytes
HIV Infections
HIV-1
T-Lymphocytes
Cell Line
Cell Differentiation
Intercellular Signaling Peptides and Proteins
Flow Cytometry
RNA-Directed DNA Polymerase
Differentiation Antigens
Thymus Gland
Interleukin-2
In Situ Hybridization
Cultured Cells
Electron Microscopy
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry
Phenotype
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Immunology

Cite this

Hatch, W. C., Tanaka, K. E., Calvelli, T., Rashbaum, W. K., Kress, Y., & Lyman, W. D. (1992). Persistent productive HIV-1 infection of a CD4- human fetal thymocyte line. Journal of Immunology, 148(10), 3055-3061.

Persistent productive HIV-1 infection of a CD4- human fetal thymocyte line. / Hatch, William C.; Tanaka, Kathryn E.; Calvelli, Theresa; Rashbaum, William K.; Kress, Yvonne; Lyman, William D.

In: Journal of Immunology, Vol. 148, No. 10, 15.05.1992, p. 3055-3061.

Research output: Contribution to journalArticle

Hatch, WC, Tanaka, KE, Calvelli, T, Rashbaum, WK, Kress, Y & Lyman, WD 1992, 'Persistent productive HIV-1 infection of a CD4- human fetal thymocyte line', Journal of Immunology, vol. 148, no. 10, pp. 3055-3061.
Hatch WC, Tanaka KE, Calvelli T, Rashbaum WK, Kress Y, Lyman WD. Persistent productive HIV-1 infection of a CD4- human fetal thymocyte line. Journal of Immunology. 1992 May 15;148(10):3055-3061.
Hatch, William C. ; Tanaka, Kathryn E. ; Calvelli, Theresa ; Rashbaum, William K. ; Kress, Yvonne ; Lyman, William D. / Persistent productive HIV-1 infection of a CD4- human fetal thymocyte line. In: Journal of Immunology. 1992 ; Vol. 148, No. 10. pp. 3055-3061.
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