Persistent Infectivity of a Disease-Associated Herpesvirus in Green Turtles after Exposure to Seawater

Sadie S. Curry, Daniel R. Brown, Jack M. Gaskin, Elliott R. Jacobson, Llewellyn M. Ehrhart, Silvia Blahak, Lawrence H. Herbst, Paul A. Klein

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Herpesviruses are associated with several diseases of marine turtles including lung-eye-trachea disease (LETD) and gray patch disease (GPD) of green turtles (Chelonia mydas) and fibropapillomatosis (FP) of green, loggerhead (Caretta caretta), and olive ridley turtles (Lepidochelys olivacea). The stability of chelonian herpesviruses in the marine environment, which may influence transmission, has not been previously studied. In these experiments, LETD-associated herpesvirus (LETV) was used as a model chelonian herpesvirus to test viral infectivity after exposure to seawater. The LETV virus preparations grown in terrapene heart (TH-1) cells were dialyzed for 24 to 120 hr against aerated artificial or natural seawater or Hank's balanced salt solution (HBBS). Fresh TH-1 cells were inoculated with dialyzed LETV, and on day 10 post-infection cells were scored for cytopathic effect. Virus samples dialyzed up to 120 hr were positive for the herpesvirus DNA polymerase gene by polymerase chain reaction. Electron microscopy revealed intact LETV nucleocapsids after exposure of LETV to artificial seawater or HBSS for 24 hr at 23 C. LETV preparations remained infectious as long as 120 hr in natural and artificial seawater at 23 C. Similar results were obtained with a second culturable chelonian herpesvirus, HV2245. LETV infectivity could not be detected after 48 hr exposvire to artificial seawater at 30 C. Since LETV and HV2245 remain infectious for extended periods of time in the marine environment, it is possible that FP-associated and GPD-associated herpesviruses also may be stable. These findings are significant both for researchers studying the epidemiological association of herpesviruses with diseases of marine turtles and for individuals who handle turtles in marine turtle conservation efforts.

Original languageEnglish (US)
Pages (from-to)792-797
Number of pages6
JournalJournal of Wildlife Diseases
Volume36
Issue number4
StatePublished - Oct 2000

Fingerprint

Chelonia mydas
Turtles
infectivity
Herpesviridae
Seawater
turtle
pathogenicity
seawater
turtles
eye disease
trachea (vertebrates)
Caretta caretta
marine environment
eyes
lungs
virus
nucleocapsid
viruses
cytopathogenicity
DNA-directed DNA polymerase

Keywords

  • Chelonia mydas
  • Disease
  • Green turtle
  • Herpesvirus
  • Infectivity
  • Seawater

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Curry, S. S., Brown, D. R., Gaskin, J. M., Jacobson, E. R., Ehrhart, L. M., Blahak, S., ... Klein, P. A. (2000). Persistent Infectivity of a Disease-Associated Herpesvirus in Green Turtles after Exposure to Seawater. Journal of Wildlife Diseases, 36(4), 792-797.

Persistent Infectivity of a Disease-Associated Herpesvirus in Green Turtles after Exposure to Seawater. / Curry, Sadie S.; Brown, Daniel R.; Gaskin, Jack M.; Jacobson, Elliott R.; Ehrhart, Llewellyn M.; Blahak, Silvia; Herbst, Lawrence H.; Klein, Paul A.

In: Journal of Wildlife Diseases, Vol. 36, No. 4, 10.2000, p. 792-797.

Research output: Contribution to journalArticle

Curry, SS, Brown, DR, Gaskin, JM, Jacobson, ER, Ehrhart, LM, Blahak, S, Herbst, LH & Klein, PA 2000, 'Persistent Infectivity of a Disease-Associated Herpesvirus in Green Turtles after Exposure to Seawater', Journal of Wildlife Diseases, vol. 36, no. 4, pp. 792-797.
Curry SS, Brown DR, Gaskin JM, Jacobson ER, Ehrhart LM, Blahak S et al. Persistent Infectivity of a Disease-Associated Herpesvirus in Green Turtles after Exposure to Seawater. Journal of Wildlife Diseases. 2000 Oct;36(4):792-797.
Curry, Sadie S. ; Brown, Daniel R. ; Gaskin, Jack M. ; Jacobson, Elliott R. ; Ehrhart, Llewellyn M. ; Blahak, Silvia ; Herbst, Lawrence H. ; Klein, Paul A. / Persistent Infectivity of a Disease-Associated Herpesvirus in Green Turtles after Exposure to Seawater. In: Journal of Wildlife Diseases. 2000 ; Vol. 36, No. 4. pp. 792-797.
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abstract = "Herpesviruses are associated with several diseases of marine turtles including lung-eye-trachea disease (LETD) and gray patch disease (GPD) of green turtles (Chelonia mydas) and fibropapillomatosis (FP) of green, loggerhead (Caretta caretta), and olive ridley turtles (Lepidochelys olivacea). The stability of chelonian herpesviruses in the marine environment, which may influence transmission, has not been previously studied. In these experiments, LETD-associated herpesvirus (LETV) was used as a model chelonian herpesvirus to test viral infectivity after exposure to seawater. The LETV virus preparations grown in terrapene heart (TH-1) cells were dialyzed for 24 to 120 hr against aerated artificial or natural seawater or Hank's balanced salt solution (HBBS). Fresh TH-1 cells were inoculated with dialyzed LETV, and on day 10 post-infection cells were scored for cytopathic effect. Virus samples dialyzed up to 120 hr were positive for the herpesvirus DNA polymerase gene by polymerase chain reaction. Electron microscopy revealed intact LETV nucleocapsids after exposure of LETV to artificial seawater or HBSS for 24 hr at 23 C. LETV preparations remained infectious as long as 120 hr in natural and artificial seawater at 23 C. Similar results were obtained with a second culturable chelonian herpesvirus, HV2245. LETV infectivity could not be detected after 48 hr exposvire to artificial seawater at 30 C. Since LETV and HV2245 remain infectious for extended periods of time in the marine environment, it is possible that FP-associated and GPD-associated herpesviruses also may be stable. These findings are significant both for researchers studying the epidemiological association of herpesviruses with diseases of marine turtles and for individuals who handle turtles in marine turtle conservation efforts.",
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