Persistent anti-inflammatory cytokine release in septic shock

Tacla Sfeir, Dhanonjoy Saha, Mark Astiz, Eric Rackow

Research output: Contribution to journalArticle

Abstract

Introduction: During septic shock dysregulation of monocyte cytokine release contributes to immunosuppression. We evaluated the difference in release of the pro-inflammatory cytokine tumor necrosis factor (TNF-α) and the anti-inflammatory cytokine interleukin 10 (IL-10) from blood monocytes isolated from healthy individuals and septic shock patients. Methods: Blood mononuclear cells from healthy volunteers (n=10, Normal) and septic shock patients (n=10, SS) were isolated and cultured. Culture plate adherent monocytes were incubated with either medium (Control) or LPS (LPS) for 18 hours at 31°C. The supernatant was collected and analyzed for TNF-α and IL-10 by ELISA. Values are mean ± SEM, * p<0.05 vs. control Results TNF- α ng/ml Normal SS Control .54±.12 .82±.25 LPS 3.26±1* 1.26±.31 IL-10 pg/ml Normal SS Control 71±26 10±4 LPS 414±170* 171±91* Conclusion: Monocytes from patients with septic shock demonstrated an attenuated secretion of the pro-inflammatory cytokine TNF-α. In contrast, the secretion of the anti-inflammatory cytokine IL-10 is unchanged. The persistence of IL-10 secretion may exacerbate the immunosuppression that develops in patients with septic shock.

Original languageEnglish (US)
Pages (from-to)A48
JournalCritical care medicine
Volume27
Issue number1 SUPPL.
DOIs
StatePublished - Jan 1 1999
Externally publishedYes

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

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