Permeability of gap junctions between embryonic cells of Fundulus: A reevaluation

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Abstract

Permeability of electrotonic junctions between isolated and reaggregated Fundulus blastomeres was evaluated with a new fluorescent dye, Lucifer yellow CH. The dye is readily shown to pass between coupled cells. It does not enter from the bathing medium, nor does it move between cells via the enlarged extracellular space sometimes seen between them. Thus, we conclude that passage is via a private pathway, presumably provided by the gap junctions described for this tissue. In contrast to previous findings, fluorescein (as the sodium salt, uranine) also passes between coupled Fundulus cells. Although it can enter from the bathing medium, it may be less concentrated in the space between a cell pair than in the uninjected cell. Again, passage via gap junctions is indicated. Molecular models demonstrate that Lucifer yellow and fluorescein are similar in size. Thus, similarity in ability to permeate junctional membranes is to be expected.

Original languageEnglish (US)
Pages (from-to)114-125
Number of pages12
JournalDevelopmental Biology
Volume65
Issue number1
DOIs
StatePublished - 1978

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Fundulidae
Gap Junctions
Permeability
Fluorescein
Blastomeres
Molecular Models
Extracellular Space
Fluorescent Dyes
Coloring Agents
Salts
Membranes

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Permeability of gap junctions between embryonic cells of Fundulus : A reevaluation. / Bennett, Michael V. L.; Spira, M. E.; Spray, David C.

In: Developmental Biology, Vol. 65, No. 1, 1978, p. 114-125.

Research output: Contribution to journalArticle

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