TY - JOUR
T1 - Peripheral effects of methionine-enkephalin on inflammatory reactions and behavior in the rat
AU - Vujić-Redžić, Vesna
AU - Dimitrijević, Mirjana
AU - Stanojević, Stanislava
AU - Kovačević-Jovanović, Vesna
AU - Miletić, Tatjana
AU - Radulović, Jelena
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2000
Y1 - 2000
N2 - Methionine-enkephalin (Met-Enk) induces notable alterations in immune and central nervous system functions. The present study was conducted in order to compare peripheral and central effects of Met-Enk on nonspecific immunity, open field behavior and pain perception in the rat. The results showed that 0.2 mg/kg of Met-Enk given intraperitoneally (i.p.) increased concanavalin A (Con-A)-induced paw edema and enhanced basal and phorbol (myristate acetate (PMA)-stimulated H2O2 production of peritoneal macrophages. Met-Enk-induced immunopotentiation was antagonized by anti-Met- Enk antibodies (anti-Met-Enk-Ig) and quaternary naltrexone (qNtx). Met-Enk injected i.p. produced an increase of horizontal and vertical locomotor activity in the open field that was reversed by i.p. administration of anti- Met-Enk-Ig and qNtx. The dose of 0.2 mg/kg of Met-Enk applied i.p. did not affect the number of writhes in the test of analgesia. Intracerebroventricular (i.c.v.) injection of Met-Enk, given in a dose that was previously shown to be immunostimulatory, enhanced only basal H2O2 production of peritoneal macrophages, and anti-Met-Enk-Ig antagonized this effect. Besides, i.c.v. treatment with anti-Met-Enk-Ig increased and decreased H2O2 production of peritoneal macrophages under basal and stimulated conditions, respectively. Met-Enk and anti-Met-Enk-Ig injected i.c.v. did not influence activity in the open field and pain sensitivity. Thus, the i.c.v. dose of Met-Enk that was sufficient to modulate immune functions did not influence behavior. It may be concluded that Met-Enk modulated non-specific immune responses and open field behavior by peripheral mechanisms. Copyright (C) 2000 S. Karger AG, Basel.
AB - Methionine-enkephalin (Met-Enk) induces notable alterations in immune and central nervous system functions. The present study was conducted in order to compare peripheral and central effects of Met-Enk on nonspecific immunity, open field behavior and pain perception in the rat. The results showed that 0.2 mg/kg of Met-Enk given intraperitoneally (i.p.) increased concanavalin A (Con-A)-induced paw edema and enhanced basal and phorbol (myristate acetate (PMA)-stimulated H2O2 production of peritoneal macrophages. Met-Enk-induced immunopotentiation was antagonized by anti-Met- Enk antibodies (anti-Met-Enk-Ig) and quaternary naltrexone (qNtx). Met-Enk injected i.p. produced an increase of horizontal and vertical locomotor activity in the open field that was reversed by i.p. administration of anti- Met-Enk-Ig and qNtx. The dose of 0.2 mg/kg of Met-Enk applied i.p. did not affect the number of writhes in the test of analgesia. Intracerebroventricular (i.c.v.) injection of Met-Enk, given in a dose that was previously shown to be immunostimulatory, enhanced only basal H2O2 production of peritoneal macrophages, and anti-Met-Enk-Ig antagonized this effect. Besides, i.c.v. treatment with anti-Met-Enk-Ig increased and decreased H2O2 production of peritoneal macrophages under basal and stimulated conditions, respectively. Met-Enk and anti-Met-Enk-Ig injected i.c.v. did not influence activity in the open field and pain sensitivity. Thus, the i.c.v. dose of Met-Enk that was sufficient to modulate immune functions did not influence behavior. It may be concluded that Met-Enk modulated non-specific immune responses and open field behavior by peripheral mechanisms. Copyright (C) 2000 S. Karger AG, Basel.
KW - Anti-methionine-enkephalin antibodies
KW - Behavior
KW - HO release
KW - Methionine-enkephalin
KW - Paw edema
KW - Peritoneal macrophages
KW - Rat
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U2 - 10.1159/000026455
DO - 10.1159/000026455
M3 - Article
C2 - 10965231
AN - SCOPUS:0033803297
SN - 1021-7401
VL - 8
SP - 70
EP - 77
JO - NeuroImmunoModulation
JF - NeuroImmunoModulation
IS - 2
ER -