Perinephric and epididymal fat affect hepatic metabolism in rats

Shani Ben-Shlomo, Francine H. Einstein, Isabel Zvibel, Dikla Atias, Amir Shlomai, Zamir Halpern, Nir Barzilai, Sigal Fishman

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The present study examined whether the perinephric and epididymal visceral fat (PEVF) depot under short-term excess nutrient protected the liver by trapping nutrient-derived nonesterified free fatty acids (NEFAs) or had deleterious effects on hepatic triglycerides (TGs) accumulation and insulin resistance due to adipokine secretion. Young rats pre-emptively underwent surgical PEVF removal or sham operations and were fed with either high-fat diet (HFD) (PEVF-HFD) or regular chow (RC) (PEVF-RC) for 3 days. Insulin sensitivity was measured by hyperinsulinemic-euglycemic clamp. Liver TG, serum NEFA, and fat-derived adipokines were assessed. Insulin and lipogenesis signaling were assessed by western blots. Pre-emptive PEVF removal significantly decreases insulin-induced suppression of hepatic glucose production (HGP) both in RC and in HFD-fed rats. In accordance with the clamp results, hepatic TG accumulation is also significantly reduced by PEVF excision both in RC and HFD-fed rats. These results are further validated by insulin signaling results, which show that pre-emptive PEVF removal increases phosphorylation of hepatic Akt, irrespective of diet. Notably, high levels of serum leptin induced by HFD are significantly reduced by pre-emptive PEVF excision. Additionally, expression of lipogenic enzyme p-acetyl-CoA-carboxylase, denoting reduced lipogenesis, is increased in the PEVF-HFD rats. In conclusion, PEVF has a deleterious effect on the liver as a source of insulin resistance-inducing adipokines irrespective of diet, and does not serve as a buffer for excess nutrients.

Original languageEnglish (US)
Pages (from-to)151-156
Number of pages6
JournalObesity
Volume20
Issue number1
DOIs
StatePublished - Jan 2012

Fingerprint

Intra-Abdominal Fat
Fats
High Fat Diet
Liver
Nonesterified Fatty Acids
Adipokines
Insulin Resistance
Lipogenesis
Triglycerides
Insulin
Food
Diet
Acetyl-CoA Carboxylase
Glucose Clamp Technique
Leptin
Serum
Buffers
Western Blotting
Phosphorylation
Glucose

ASJC Scopus subject areas

  • Endocrinology
  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Ben-Shlomo, S., Einstein, F. H., Zvibel, I., Atias, D., Shlomai, A., Halpern, Z., ... Fishman, S. (2012). Perinephric and epididymal fat affect hepatic metabolism in rats. Obesity, 20(1), 151-156. https://doi.org/10.1038/oby.2011.261

Perinephric and epididymal fat affect hepatic metabolism in rats. / Ben-Shlomo, Shani; Einstein, Francine H.; Zvibel, Isabel; Atias, Dikla; Shlomai, Amir; Halpern, Zamir; Barzilai, Nir; Fishman, Sigal.

In: Obesity, Vol. 20, No. 1, 01.2012, p. 151-156.

Research output: Contribution to journalArticle

Ben-Shlomo, S, Einstein, FH, Zvibel, I, Atias, D, Shlomai, A, Halpern, Z, Barzilai, N & Fishman, S 2012, 'Perinephric and epididymal fat affect hepatic metabolism in rats', Obesity, vol. 20, no. 1, pp. 151-156. https://doi.org/10.1038/oby.2011.261
Ben-Shlomo S, Einstein FH, Zvibel I, Atias D, Shlomai A, Halpern Z et al. Perinephric and epididymal fat affect hepatic metabolism in rats. Obesity. 2012 Jan;20(1):151-156. https://doi.org/10.1038/oby.2011.261
Ben-Shlomo, Shani ; Einstein, Francine H. ; Zvibel, Isabel ; Atias, Dikla ; Shlomai, Amir ; Halpern, Zamir ; Barzilai, Nir ; Fishman, Sigal. / Perinephric and epididymal fat affect hepatic metabolism in rats. In: Obesity. 2012 ; Vol. 20, No. 1. pp. 151-156.
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