Performance evaluation of the new ADVIA Centaur system cyclosporine assay (single-step extraction).

Steven J. Soldin, Robert W. Hardy, Frank H. Wians, Jody A. Balko, Damodara R. Mendu, Carolyn H. Chaffin, Frank Streit, Minas Barbarakis, Julie Martel, William Canfield, H. Roma Levy, Michael Oellerich

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Cyclosporine (CsA) monitoring is essential for transplant success. We report a performance study of the recently released, fully automated Siemens ADVIA Centaur CsA assay. Whole blood samples from 248 transplant patients were prepared using a new 1-step extraction method. Performance evaluations vs. HPLC-tandem MS (LC-MS/MS), Abbott TDx and AxSYM assays were conducted according to CLSI EP5-A2 and EP9-A2 guidelines. The correlation coefficient for LC-MS/MS and ADVIA Centaur was > or = 0.97 at each site, and for each transplant type. Regression analysis yielded y=0.94x+19 for all sites: 95% CI=0.91-0.96 (slope) and 10-28 (intercept). Absolute and relative bias was minimal for C0 and C2 sampling. Centaur vs. Abbott TDx and AxSYM assays: y = 0.72x+6, r = 0.98, 95% CI = 0.70-0.73 (slope), 3-9 (intercept); and y = 0.69x+18, r = 0.97, 95% CI = 0.67-0.71 (slope), 8-27(intercept). Within run CVs were 4.5%-7.1%, total CVs were 5.3%-7.7%. The ADVIA Centaur assay compared favorably with LC-MS/MS and Abbott assays, displaying good correlation for all transplant types and methods.

Original languageEnglish (US)
Pages (from-to)806-811
Number of pages6
JournalClinica chimica acta; international journal of clinical chemistry
Volume411
Issue number11-12
StatePublished - Jun 3 2010

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

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    Soldin, S. J., Hardy, R. W., Wians, F. H., Balko, J. A., Mendu, D. R., Chaffin, C. H., Streit, F., Barbarakis, M., Martel, J., Canfield, W., Levy, H. R., & Oellerich, M. (2010). Performance evaluation of the new ADVIA Centaur system cyclosporine assay (single-step extraction). Clinica chimica acta; international journal of clinical chemistry, 411(11-12), 806-811.