Peptidomics

Identification and quantification of endogenous peptides in neuroendocrine tissues

Lloyd D. Fricker, Jlhyeon Lim, Hui Pan, Fa Yun Che

Research output: Contribution to journalArticle

151 Citations (Scopus)

Abstract

Neuropeptides perform a large variety of functions as inter-cellular signaling molecules. While most proteomic studies involve digestion of the proteins with trypsin or other proteases, peptidomics studies usually analyze the native peptide forms. Neuropeptides can be studied by using mass spectrometry for identification and quantitation. In many cases, mass spectrometry provides an understanding of the precise molecular form of the native peptide, including post-translational cleavages and other modifications. Quantitative peptidomics studies generally use differential isotopic tags to label two sets of extracted peptides, as done with proteomic studies, except that the Cys-based reagents typically used for quantitation of proteins are not suitable because most peptides lack Cys residues. Instead, a number of amine-specific labels have been created and some of these are useful for peptide quantitation by mass spectrometry. In this review, peptidomics techniques are discussed along with the major findings of many recent studies and future directions for the field.

Original languageEnglish (US)
Pages (from-to)327-344
Number of pages18
JournalMass Spectrometry Reviews
Volume25
Issue number2
DOIs
StatePublished - Mar 2006

Fingerprint

peptides
Tissue
Peptides
Mass spectrometry
Mass Spectrometry
mass spectroscopy
Neuropeptides
Proteomics
Labels
Cell signaling
proteins
trypsin
protease
Trypsin
Proteolysis
Amines
reagents
cleavage
amines
Proteins

Keywords

  • Carboxypeptidase
  • Neuropeptide
  • Peptide processing
  • Prohormone convertase

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Peptidomics : Identification and quantification of endogenous peptides in neuroendocrine tissues. / Fricker, Lloyd D.; Lim, Jlhyeon; Pan, Hui; Che, Fa Yun.

In: Mass Spectrometry Reviews, Vol. 25, No. 2, 03.2006, p. 327-344.

Research output: Contribution to journalArticle

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