Pentapeptide repeat proteins

Matthew W. Vetting, Subray Hegde, Jorge E. Fajardo, Andras Fiser, Steven L. Roderick, Howard E. Takiff, John S. Blanchard

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

The pentapeptide repeat protein (PRP) family has more than 500 members in the prokaryotic and eukaryotic kingdoms. These proteins are composed of, or contain domains composed of, tandemly repeated amino acid sequences with a consensus sequence of [S,T,A,V][D,N][L,F][S,T,R][G]. The biochemical function of the vast majority of PRP family members is unknown. The three-dimensional structure of the first member of the PRP family was determined for the fluoroquinolone resistance protein (MfpA) from Mycobacterium tuberculosis. The structure revealed that the pentapeptide repeats encode the folding of a novel right-handed quadrilateral β-helix. MfpA binds to DNA gyrase and inhibits its activity. The rod-shaped, dimeric protein exhibits remarkable similarity in size, shape, and electrostatics to DNA.

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalBiochemistry
Volume45
Issue number1
DOIs
StatePublished - Jan 10 2006

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Proteins
DNA Gyrase
Fluoroquinolones
Consensus Sequence
Static Electricity
Mycobacterium tuberculosis
Amino Acid Sequence
Electrostatics
Amino Acids
DNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

Pentapeptide repeat proteins. / Vetting, Matthew W.; Hegde, Subray; Fajardo, Jorge E.; Fiser, Andras; Roderick, Steven L.; Takiff, Howard E.; Blanchard, John S.

In: Biochemistry, Vol. 45, No. 1, 10.01.2006, p. 1-10.

Research output: Contribution to journalArticle

Vetting MW, Hegde S, Fajardo JE, Fiser A, Roderick SL, Takiff HE et al. Pentapeptide repeat proteins. Biochemistry. 2006 Jan 10;45(1):1-10. https://doi.org/10.1021/bi052130w
Vetting, Matthew W. ; Hegde, Subray ; Fajardo, Jorge E. ; Fiser, Andras ; Roderick, Steven L. ; Takiff, Howard E. ; Blanchard, John S. / Pentapeptide repeat proteins. In: Biochemistry. 2006 ; Vol. 45, No. 1. pp. 1-10.
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