PEGylation of a a-Hb using succinimidyl propionic acid PEG 5K: Conjugation chemistry and PEG shell structure dictate respectively the oxygen affi nity and resuscitation fl uid like properties of PEG a a-Hbs

Fantao Meng; Amy G. Tsai; Marcos Intaglietta; Seetharama A. Acharya

doi:10.3109/21691401.2014.885443

PEGylation of a a-Hb using succinimidyl propionic acid PEG 5K: Conjugation chemistry and PEG shell structure dictate respectively the oxygen affi nity and resuscitation fl uid like properties of PEG a a-Hbs

Fantao Meng, Amy G. Tsai, Marcos Intaglietta, Seetharama A. Acharya

Medicine

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

PEGylation of intramolecularly crosslinked Hb has been studied here to overcome the limitation of dissociation of Hb tetramers. New hexa and deca PEGylated low oxygen affi nity PEG-a a Hbs have been generated. Influences of PEG conjugation chemistry and the PEG shell structure on the functional properties as well as PEGylation induced plasma expander like properties of the protein has been delineated. The results have established that in the design of PEG-Hbs as oxygen therapeutics, the Influences of conjugation chemistry and the PEG shell structure on the oxygen affi nity of Hb needs to be optimized independently besides optimizing the PEG shell structure for inducing resuscitation fl uid like properties.

Original language	English (US)
Pages (from-to)	270-281
Number of pages	12
Journal	Artificial Cells, Nanomedicine and Biotechnology
Volume	43
Issue number	4
DOIs	https://doi.org/10.3109/21691401.2014.885443
State	Published - 2015

Keywords

Blood substitute
PEG shell
PEGylated hemoglobin
PEGylation
oxygen affi nity
plasma expander

ASJC Scopus subject areas

Biotechnology
Medicine (miscellaneous)
Biomedical Engineering
Pharmaceutical Science

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Meng, F., Tsai, A. G., Intaglietta, M., & Acharya, S. A. (2015). PEGylation of a a-Hb using succinimidyl propionic acid PEG 5K: Conjugation chemistry and PEG shell structure dictate respectively the oxygen affi nity and resuscitation fl uid like properties of PEG a a-Hbs. Artificial Cells, Nanomedicine and Biotechnology, 43(4), 270-281. https://doi.org/10.3109/21691401.2014.885443

PEGylation of a a-Hb using succinimidyl propionic acid PEG 5K: Conjugation chemistry and PEG shell structure dictate respectively the oxygen affi nity and resuscitation fl uid like properties of PEG a a-Hbs. / Meng, Fantao; Tsai, Amy G.; Intaglietta, Marcos et al.
In: Artificial Cells, Nanomedicine and Biotechnology, Vol. 43, No. 4, 2015, p. 270-281.

Research output: Contribution to journal › Article › peer-review

Meng, F, Tsai, AG, Intaglietta, M & Acharya, SA 2015, 'PEGylation of a a-Hb using succinimidyl propionic acid PEG 5K: Conjugation chemistry and PEG shell structure dictate respectively the oxygen affi nity and resuscitation fl uid like properties of PEG a a-Hbs', Artificial Cells, Nanomedicine and Biotechnology, vol. 43, no. 4, pp. 270-281. https://doi.org/10.3109/21691401.2014.885443

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abstract = "PEGylation of intramolecularly crosslinked Hb has been studied here to overcome the limitation of dissociation of Hb tetramers. New hexa and deca PEGylated low oxygen affi nity PEG-a a Hbs have been generated. Influences of PEG conjugation chemistry and the PEG shell structure on the functional properties as well as PEGylation induced plasma expander like properties of the protein has been delineated. The results have established that in the design of PEG-Hbs as oxygen therapeutics, the Influences of conjugation chemistry and the PEG shell structure on the oxygen affi nity of Hb needs to be optimized independently besides optimizing the PEG shell structure for inducing resuscitation fl uid like properties.",

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PEGylation of a a-Hb using succinimidyl propionic acid PEG 5K: Conjugation chemistry and PEG shell structure dictate respectively the oxygen affi nity and resuscitation fl uid like properties of PEG a a-Hbs

Abstract

Keywords

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