PEGylation of a a-Hb using succinimidyl propionic acid PEG 5K: Conjugation chemistry and PEG shell structure dictate respectively the oxygen affi nity and resuscitation fl uid like properties of PEG a a-Hbs

Fantao Meng, Amy G. Tsai, Marcos Intaglietta, Seetharama A. Acharya

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

PEGylation of intramolecularly crosslinked Hb has been studied here to overcome the limitation of dissociation of Hb tetramers. New hexa and deca PEGylated low oxygen affi nity PEG-a a Hbs have been generated. Influences of PEG conjugation chemistry and the PEG shell structure on the functional properties as well as PEGylation induced plasma expander like properties of the protein has been delineated. The results have established that in the design of PEG-Hbs as oxygen therapeutics, the Influences of conjugation chemistry and the PEG shell structure on the oxygen affi nity of Hb needs to be optimized independently besides optimizing the PEG shell structure for inducing resuscitation fl uid like properties.

Original languageEnglish (US)
Pages (from-to)270-281
Number of pages12
JournalArtificial Cells, Nanomedicine and Biotechnology
Volume43
Issue number4
DOIs
StatePublished - 2015

Fingerprint

Resuscitation
Propionic acid
Polyethylene glycols
Oxygen
propionic acid
Proteins
Plasmas
Therapeutics

Keywords

  • Blood substitute
  • oxygen affi nity
  • PEG shell
  • PEGylated hemoglobin
  • PEGylation
  • plasma expander

ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Pharmaceutical Science

Cite this

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title = "PEGylation of a a-Hb using succinimidyl propionic acid PEG 5K: Conjugation chemistry and PEG shell structure dictate respectively the oxygen affi nity and resuscitation fl uid like properties of PEG a a-Hbs",
abstract = "PEGylation of intramolecularly crosslinked Hb has been studied here to overcome the limitation of dissociation of Hb tetramers. New hexa and deca PEGylated low oxygen affi nity PEG-a a Hbs have been generated. Influences of PEG conjugation chemistry and the PEG shell structure on the functional properties as well as PEGylation induced plasma expander like properties of the protein has been delineated. The results have established that in the design of PEG-Hbs as oxygen therapeutics, the Influences of conjugation chemistry and the PEG shell structure on the oxygen affi nity of Hb needs to be optimized independently besides optimizing the PEG shell structure for inducing resuscitation fl uid like properties.",
keywords = "Blood substitute, oxygen affi nity, PEG shell, PEGylated hemoglobin, PEGylation, plasma expander",
author = "Fantao Meng and Tsai, {Amy G.} and Marcos Intaglietta and Acharya, {Seetharama A.}",
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T1 - PEGylation of a a-Hb using succinimidyl propionic acid PEG 5K

T2 - Conjugation chemistry and PEG shell structure dictate respectively the oxygen affi nity and resuscitation fl uid like properties of PEG a a-Hbs

AU - Meng, Fantao

AU - Tsai, Amy G.

AU - Intaglietta, Marcos

AU - Acharya, Seetharama A.

PY - 2015

Y1 - 2015

N2 - PEGylation of intramolecularly crosslinked Hb has been studied here to overcome the limitation of dissociation of Hb tetramers. New hexa and deca PEGylated low oxygen affi nity PEG-a a Hbs have been generated. Influences of PEG conjugation chemistry and the PEG shell structure on the functional properties as well as PEGylation induced plasma expander like properties of the protein has been delineated. The results have established that in the design of PEG-Hbs as oxygen therapeutics, the Influences of conjugation chemistry and the PEG shell structure on the oxygen affi nity of Hb needs to be optimized independently besides optimizing the PEG shell structure for inducing resuscitation fl uid like properties.

AB - PEGylation of intramolecularly crosslinked Hb has been studied here to overcome the limitation of dissociation of Hb tetramers. New hexa and deca PEGylated low oxygen affi nity PEG-a a Hbs have been generated. Influences of PEG conjugation chemistry and the PEG shell structure on the functional properties as well as PEGylation induced plasma expander like properties of the protein has been delineated. The results have established that in the design of PEG-Hbs as oxygen therapeutics, the Influences of conjugation chemistry and the PEG shell structure on the oxygen affi nity of Hb needs to be optimized independently besides optimizing the PEG shell structure for inducing resuscitation fl uid like properties.

KW - Blood substitute

KW - oxygen affi nity

KW - PEG shell

KW - PEGylated hemoglobin

KW - PEGylation

KW - plasma expander

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