PCSK7 genotype modifies effect of a weight-loss diet on 2-year changes of insulin resistance: the POUNDS LOST trial

Tao Huang, Jinyan Huang, Qibin Qi, Yanping Li, George A. Bray, Jennifer Rood, Frank M. Sacks, Lu Qi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

OBJECTIVE: A common variant rs236918 in the PCSK7 gene has the strongest association with iron homeostasis and is related to insulin resistance. Dietary carbohydrate (CHO) modulates the genetic effect on insulin resistance. We examined whether 2-year weight-loss diets modify the effect of PCSK7 genetic variants on changes in fasting insulin levels and insulin resistance in a randomized, controlled trial.

RESEARCH DESIGN AND METHODS: Data were analyzed in the Preventing Overweight Using Novel Dietary Strategies (POUNDS LOST) trial, which is a randomized, controlled 2-year weight-loss trial using diets that differed in macronutrient proportions. PCSK7 rs236918 was genotyped in 730 overweight or obese adults (80% whites) in this trial. We assessed the progression in fasting insulin and glucose levels, and insulin resistance by genotypes.

RESULTS: During the 6-month weight-loss phase, the PCSK7 rs236918 G allele was significantly associated with greater decreases in fasting insulin levels in the high-dietary CHO group (P for interaction = 0.04), while the interaction for changes in HOMA-insulin resistance (HOMA-IR) (P for interaction = 0.06) did not reach significant levels in white subjects. The G allele was significantly associated with a greater decrease in fasting insulin levels and HOMA-IR in response to high dietary CHO levels (P = 0.02 and P = 0.03, respectively). From 6 months to 2 years (weight-regain phase), the interactions became attenuated due to the regaining of weight (P for interactions = 0.08 and 0.06, respectively). In addition, we observed similar and even stronger results in the whole-study samples from the trial.

CONCLUSIONS: Our data suggest that PCSK7 genotypes may interact with dietary CHO intake on changes in insulin sensitivity in the white Americans.

Original languageEnglish (US)
Pages (from-to)439-444
Number of pages6
JournalDiabetes Care
Volume38
Issue number3
DOIs
StatePublished - Mar 1 2015
Externally publishedYes

Fingerprint

Reducing Diet
Insulin Resistance
Genotype
Fasting
Insulin
Weight Loss
Alleles
Dietary Carbohydrates
Weights and Measures
Homeostasis
Iron
Randomized Controlled Trials
Diet
Glucose

ASJC Scopus subject areas

  • Medicine(all)

Cite this

PCSK7 genotype modifies effect of a weight-loss diet on 2-year changes of insulin resistance : the POUNDS LOST trial. / Huang, Tao; Huang, Jinyan; Qi, Qibin; Li, Yanping; Bray, George A.; Rood, Jennifer; Sacks, Frank M.; Qi, Lu.

In: Diabetes Care, Vol. 38, No. 3, 01.03.2015, p. 439-444.

Research output: Contribution to journalArticle

Huang, Tao ; Huang, Jinyan ; Qi, Qibin ; Li, Yanping ; Bray, George A. ; Rood, Jennifer ; Sacks, Frank M. ; Qi, Lu. / PCSK7 genotype modifies effect of a weight-loss diet on 2-year changes of insulin resistance : the POUNDS LOST trial. In: Diabetes Care. 2015 ; Vol. 38, No. 3. pp. 439-444.
@article{342fb4d830fe43a1b0d14174c7a74146,
title = "PCSK7 genotype modifies effect of a weight-loss diet on 2-year changes of insulin resistance: the POUNDS LOST trial",
abstract = "OBJECTIVE: A common variant rs236918 in the PCSK7 gene has the strongest association with iron homeostasis and is related to insulin resistance. Dietary carbohydrate (CHO) modulates the genetic effect on insulin resistance. We examined whether 2-year weight-loss diets modify the effect of PCSK7 genetic variants on changes in fasting insulin levels and insulin resistance in a randomized, controlled trial.RESEARCH DESIGN AND METHODS: Data were analyzed in the Preventing Overweight Using Novel Dietary Strategies (POUNDS LOST) trial, which is a randomized, controlled 2-year weight-loss trial using diets that differed in macronutrient proportions. PCSK7 rs236918 was genotyped in 730 overweight or obese adults (80{\%} whites) in this trial. We assessed the progression in fasting insulin and glucose levels, and insulin resistance by genotypes.RESULTS: During the 6-month weight-loss phase, the PCSK7 rs236918 G allele was significantly associated with greater decreases in fasting insulin levels in the high-dietary CHO group (P for interaction = 0.04), while the interaction for changes in HOMA-insulin resistance (HOMA-IR) (P for interaction = 0.06) did not reach significant levels in white subjects. The G allele was significantly associated with a greater decrease in fasting insulin levels and HOMA-IR in response to high dietary CHO levels (P = 0.02 and P = 0.03, respectively). From 6 months to 2 years (weight-regain phase), the interactions became attenuated due to the regaining of weight (P for interactions = 0.08 and 0.06, respectively). In addition, we observed similar and even stronger results in the whole-study samples from the trial.CONCLUSIONS: Our data suggest that PCSK7 genotypes may interact with dietary CHO intake on changes in insulin sensitivity in the white Americans.",
author = "Tao Huang and Jinyan Huang and Qibin Qi and Yanping Li and Bray, {George A.} and Jennifer Rood and Sacks, {Frank M.} and Lu Qi",
year = "2015",
month = "3",
day = "1",
doi = "10.2337/dc14-0473",
language = "English (US)",
volume = "38",
pages = "439--444",
journal = "Diabetes Care",
issn = "1935-5548",
publisher = "American Diabetes Association Inc.",
number = "3",

}

TY - JOUR

T1 - PCSK7 genotype modifies effect of a weight-loss diet on 2-year changes of insulin resistance

T2 - the POUNDS LOST trial

AU - Huang, Tao

AU - Huang, Jinyan

AU - Qi, Qibin

AU - Li, Yanping

AU - Bray, George A.

AU - Rood, Jennifer

AU - Sacks, Frank M.

AU - Qi, Lu

PY - 2015/3/1

Y1 - 2015/3/1

N2 - OBJECTIVE: A common variant rs236918 in the PCSK7 gene has the strongest association with iron homeostasis and is related to insulin resistance. Dietary carbohydrate (CHO) modulates the genetic effect on insulin resistance. We examined whether 2-year weight-loss diets modify the effect of PCSK7 genetic variants on changes in fasting insulin levels and insulin resistance in a randomized, controlled trial.RESEARCH DESIGN AND METHODS: Data were analyzed in the Preventing Overweight Using Novel Dietary Strategies (POUNDS LOST) trial, which is a randomized, controlled 2-year weight-loss trial using diets that differed in macronutrient proportions. PCSK7 rs236918 was genotyped in 730 overweight or obese adults (80% whites) in this trial. We assessed the progression in fasting insulin and glucose levels, and insulin resistance by genotypes.RESULTS: During the 6-month weight-loss phase, the PCSK7 rs236918 G allele was significantly associated with greater decreases in fasting insulin levels in the high-dietary CHO group (P for interaction = 0.04), while the interaction for changes in HOMA-insulin resistance (HOMA-IR) (P for interaction = 0.06) did not reach significant levels in white subjects. The G allele was significantly associated with a greater decrease in fasting insulin levels and HOMA-IR in response to high dietary CHO levels (P = 0.02 and P = 0.03, respectively). From 6 months to 2 years (weight-regain phase), the interactions became attenuated due to the regaining of weight (P for interactions = 0.08 and 0.06, respectively). In addition, we observed similar and even stronger results in the whole-study samples from the trial.CONCLUSIONS: Our data suggest that PCSK7 genotypes may interact with dietary CHO intake on changes in insulin sensitivity in the white Americans.

AB - OBJECTIVE: A common variant rs236918 in the PCSK7 gene has the strongest association with iron homeostasis and is related to insulin resistance. Dietary carbohydrate (CHO) modulates the genetic effect on insulin resistance. We examined whether 2-year weight-loss diets modify the effect of PCSK7 genetic variants on changes in fasting insulin levels and insulin resistance in a randomized, controlled trial.RESEARCH DESIGN AND METHODS: Data were analyzed in the Preventing Overweight Using Novel Dietary Strategies (POUNDS LOST) trial, which is a randomized, controlled 2-year weight-loss trial using diets that differed in macronutrient proportions. PCSK7 rs236918 was genotyped in 730 overweight or obese adults (80% whites) in this trial. We assessed the progression in fasting insulin and glucose levels, and insulin resistance by genotypes.RESULTS: During the 6-month weight-loss phase, the PCSK7 rs236918 G allele was significantly associated with greater decreases in fasting insulin levels in the high-dietary CHO group (P for interaction = 0.04), while the interaction for changes in HOMA-insulin resistance (HOMA-IR) (P for interaction = 0.06) did not reach significant levels in white subjects. The G allele was significantly associated with a greater decrease in fasting insulin levels and HOMA-IR in response to high dietary CHO levels (P = 0.02 and P = 0.03, respectively). From 6 months to 2 years (weight-regain phase), the interactions became attenuated due to the regaining of weight (P for interactions = 0.08 and 0.06, respectively). In addition, we observed similar and even stronger results in the whole-study samples from the trial.CONCLUSIONS: Our data suggest that PCSK7 genotypes may interact with dietary CHO intake on changes in insulin sensitivity in the white Americans.

UR - http://www.scopus.com/inward/record.url?scp=84941112168&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84941112168&partnerID=8YFLogxK

U2 - 10.2337/dc14-0473

DO - 10.2337/dc14-0473

M3 - Article

C2 - 25504030

AN - SCOPUS:84941112168

VL - 38

SP - 439

EP - 444

JO - Diabetes Care

JF - Diabetes Care

SN - 1935-5548

IS - 3

ER -