PCR detection of human papillomavirus

Comparison between MY09/MY11 and GP5+/GP6+ primer systems

Weimin Qu, Gang Jiang, Yvette Cruz, Chee Jen Chang, Gloria Y F Ho, Robert S. Klein, Robert D. Burk

Research output: Contribution to journalArticle

361 Citations (Scopus)

Abstract

Human papillomavirus (HPV) is an etiologic agent of cervical cancer and is the must common sexually transmitted disease in women. PCR amplification of HPV genomes is the most sensitive method for the detection of cervicovaginal HPV. We have compared the two most commonly used PCR primer sets, MY09/MY11 (MY-PCR) and GP5+/GP6+ (GP+-PCR), for the detection of HPV DNA in cervicovaginal lavage samples from 208 women. Oligonucleotide probes for 39 different HPV types were used. Both primer sets amplified a wide spectrum of HPV genotypes and detected similar overall prevalences of 45% (94 of 208) and 43% (89 of 208), respectively. The MY-PCR system detected 27 of 30 (90%) samples with multiple HPV types, whereas the GP+-PCR system detected 14 of 30 (47%) samples with multiple HPV types. Differences in the detection of HPV types 35, 53, and 61 were noted between the two primer systems. Serial dilution of plasmid templates indicated a 3-log decrease in the amplification of HPV type 35 by MY-PCR and HPV types 53 and 61 by GP+-PCR. These results indicate that although the MY-PCR and GP+-PCR identified nearly equivalent prevalences of HPV in a set of clinical samples, differences in the detection of specific types and infections with multiple types were found. Differences in the sensitivities and characteristics of the PCR systems for the detection of HPV within clinical samples should be considered when comparing data between studies and/or in designing new studies or clinical trials.

Original languageEnglish (US)
Pages (from-to)1304-1310
Number of pages7
JournalJournal of Clinical Microbiology
Volume35
Issue number6
StatePublished - Jun 1997

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Polymerase Chain Reaction
Oligonucleotide Probes
Therapeutic Irrigation
Human Genome
Sexually Transmitted Diseases
Uterine Cervical Neoplasms
Plasmids
Genotype
Clinical Trials
DNA
Infection

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Qu, W., Jiang, G., Cruz, Y., Chang, C. J., Ho, G. Y. F., Klein, R. S., & Burk, R. D. (1997). PCR detection of human papillomavirus: Comparison between MY09/MY11 and GP5+/GP6+ primer systems. Journal of Clinical Microbiology, 35(6), 1304-1310.

PCR detection of human papillomavirus : Comparison between MY09/MY11 and GP5+/GP6+ primer systems. / Qu, Weimin; Jiang, Gang; Cruz, Yvette; Chang, Chee Jen; Ho, Gloria Y F; Klein, Robert S.; Burk, Robert D.

In: Journal of Clinical Microbiology, Vol. 35, No. 6, 06.1997, p. 1304-1310.

Research output: Contribution to journalArticle

Qu, W, Jiang, G, Cruz, Y, Chang, CJ, Ho, GYF, Klein, RS & Burk, RD 1997, 'PCR detection of human papillomavirus: Comparison between MY09/MY11 and GP5+/GP6+ primer systems', Journal of Clinical Microbiology, vol. 35, no. 6, pp. 1304-1310.
Qu W, Jiang G, Cruz Y, Chang CJ, Ho GYF, Klein RS et al. PCR detection of human papillomavirus: Comparison between MY09/MY11 and GP5+/GP6+ primer systems. Journal of Clinical Microbiology. 1997 Jun;35(6):1304-1310.
Qu, Weimin ; Jiang, Gang ; Cruz, Yvette ; Chang, Chee Jen ; Ho, Gloria Y F ; Klein, Robert S. ; Burk, Robert D. / PCR detection of human papillomavirus : Comparison between MY09/MY11 and GP5+/GP6+ primer systems. In: Journal of Clinical Microbiology. 1997 ; Vol. 35, No. 6. pp. 1304-1310.
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