@article{9aa654c3bac64615a382029650d899b3,
title = "PCF4 encodes an RNA polymerase III transcription factor with homology to TFIIB",
abstract = "A dominant mutation in the PCF4 gene of S. cerevisiae was isolated as a suppressor of a tRNA gene A block promoter mutation. In vitro studies indicate that PCF4 is a stoichiometrically-required RNA polymerase III (pol III) transcription initiation factor. We show that the PCF4-1 mutation increases the number of transcriptionally competent preinitiation complexes by affecting a limiting activity in yeast cell extracts that is squelched by excess TFIIIC. The PCF4 gene encodes a TFIIB homolog whose size, biochemical, and genetic properties are consistent with those of the 70 kd subunit of TFIIIB. The TFIIB homology of PCF4 suggests a means for determining the polymerase specificity of a gene.",
author = "Alfredo L{\'o}pez-De-Le{\'o}n and Monett Librizzi and Karen Puglia and Willis, {Ian M.}",
note = "Funding Information: We are very grateful to Steve Buratowski for providing the sequence of the 7094 gene prior to publication and for the YEp24 plasmid containing this gene. Thanks also to Jon Warner, LaDonne Schulman, and lndra Sethy for helpful comments on the manuscript. This work was supported by grants to I. M. W. from the National Institutes of Health (GM42728) and the Alexandrine and Alexander Sinsheimer Fund and BRSG funds to the Albert Einstein College of Medicine. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 USC Section 1734 solely to indicate this fact.",
year = "1992",
month = oct,
day = "16",
doi = "10.1016/0092-8674(92)90350-L",
language = "English (US)",
volume = "71",
pages = "211--220",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "2",
}