Patterns of expression of factor VIII and von Willebrand factor by endothelial cell subsets in vivo

Junliang Pan, Thanh Theresa Dinh, Anusha Rajaraman, Mike Lee, Alexander Scholz, Cathrin J. Czupalla, Helena Kiefel, Li Zhu, Lijun Xia, John Morser, Haiyan Jiang, Laura Santambrogio, Eugene C. Butcher

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Circulating factor VIII (FVIII) is derived from liver and from extrahepatic sources probably of endothelial origin, but the vascular sites of FVIII production remain unclear. Among organs profiled, only liver and lymph nodes (LNs) show abundant expression of F8 messenger RNA (mRNA). Transcriptomic profiling of subsets of stromal cells, including endothelial cells (ECs) from mouse LNs and other tissues, showed that F8 mRNA is expressed by lymphatic ECs (LECs) but not by capillary ECs (capECs), fibroblastic reticular cells, or hematopoietic cells. Among blood ECs profiled, F8 expression was seen only in fenestrated ECs (liver sinusoidal and renal glomerular ECs) and some high endothelial venules. In contrast, von Willebrand factor mRNA was expressed in capECs but not in LECs; it was coexpressed with F8 mRNA in postcapillary high endothelial venules. Purified LECs and liver sinusoidal ECs but not capECs from LNs secrete active FVIII in culture, and human and mouse lymph contained substantial FVIII:C activity. Our results revealed localized vascular expression of FVIII and von Willebrand factor and identified LECs as a major cellular source of FVIII in extrahepatic tissues.

Original languageEnglish (US)
Pages (from-to)104-109
Number of pages6
JournalBlood
Volume128
Issue number1
DOIs
StatePublished - Jul 7 2016

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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    Pan, J., Dinh, T. T., Rajaraman, A., Lee, M., Scholz, A., Czupalla, C. J., Kiefel, H., Zhu, L., Xia, L., Morser, J., Jiang, H., Santambrogio, L., & Butcher, E. C. (2016). Patterns of expression of factor VIII and von Willebrand factor by endothelial cell subsets in vivo. Blood, 128(1), 104-109. https://doi.org/10.1182/blood-2015-12-684688