Pathogenic anti-DNA antibodies modulate gene expression in mesangial cells

Involvement of HMGB1 in anti-DNA antibody-induced renal injury

Xiaoping Qing, Milena Pitashny, David B. Thomas, Franck J. Barrat, Mark P. Hogarth, Chaim Putterman

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Although anti-DNA antibodies have been decisively linked to the pathogenesis of lupus nephritis, the mechanisms have not been conclusively determined. Recently, we reported that anti-DNA antibodies may contribute to kidney damage by upregulation of proinflammatory genes in mesangial cells (MC), a process involving both Fc receptor-dependent and independent pathways. In investigating the mechanism by which pathogenic anti-DNA antibodies modulate gene expression in MC, we found that the pathogenic anti-DNA antibody 1A3F bound to high mobility group binding protein 1 (HMGB1), an endogenous ligand for TLR2/4 and RAGE (receptor for advanced glycation end products). Interestingly, HMGB1 treatment of MC induced a similar pattern of genes as stimulation with 1A3F. Furthermore, HMGB1 and 1A3F exhibited a synergistic proinflammatory effect in the kidney, where increased expression of HMGB1 was found in lupus patients but not in patients with other types of renal disease. TLR2/Fc and RAGE/Fc inhibited the proinflammatory effects of 1A3F on MC. Finally, we found enhanced susceptibility of lupus prone MRL-lpr/lpr (MRL/lpr) as compared to normal BALB/c derived MC to pathogenic anti-DNA antibody and LPS stimulation (in particular enhanced chemokine synthesis), in addition to significantly increased expression of TLR4. Our results suggest that gene upregulation in MC induced by nephritogenic anti-DNA antibodies is TLR2/4 and RAGE-dependent. Finally, HMGB1 may act as a proinflammatory mediator in antibody-induced kidney damage in systemic lupus erythematosus (SLE).

Original languageEnglish (US)
Pages (from-to)61-73
Number of pages13
JournalImmunology Letters
Volume121
Issue number1
DOIs
StatePublished - Nov 16 2008

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High Mobility Group Proteins
Mesangial Cells
Antinuclear Antibodies
Carrier Proteins
Kidney
Gene Expression
Toll-Like Receptor 2
Wounds and Injuries
Fc Receptors
Up-Regulation
Genes
Lupus Nephritis
Chemokines
Systemic Lupus Erythematosus
Ligands
Antibodies
Advanced Glycosylation End Product-Specific Receptor

Keywords

  • Autoantibodies
  • Inflammation
  • Systemic lupus erythematosus

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Pathogenic anti-DNA antibodies modulate gene expression in mesangial cells : Involvement of HMGB1 in anti-DNA antibody-induced renal injury. / Qing, Xiaoping; Pitashny, Milena; Thomas, David B.; Barrat, Franck J.; Hogarth, Mark P.; Putterman, Chaim.

In: Immunology Letters, Vol. 121, No. 1, 16.11.2008, p. 61-73.

Research output: Contribution to journalArticle

Qing, Xiaoping ; Pitashny, Milena ; Thomas, David B. ; Barrat, Franck J. ; Hogarth, Mark P. ; Putterman, Chaim. / Pathogenic anti-DNA antibodies modulate gene expression in mesangial cells : Involvement of HMGB1 in anti-DNA antibody-induced renal injury. In: Immunology Letters. 2008 ; Vol. 121, No. 1. pp. 61-73.
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