Patch-clamp recordings and calcium imaging followed by single-cell PCR reveal the developmental profile of 13 genes in iPSC-derived human neurons

Glenn S. Belinsky, Matthew T. Rich, Carissa L. Sirois, Shaina M. Short, Erika Pedrosa, Herbert M. Lachman, Srdjan D. Antic

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Molecular genetic studies are typically performed on homogenized biological samples, resulting in contamination from non-neuronal cells. To improve expression profiling of neurons we combined patch recordings with single-cell PCR. Two iPSC lines (healthy subject and 22q11.2 deletion) were differentiated into neurons. Patch electrode recordings were performed on 229 human cells from Day-13 to Day-88, followed by capture and single-cell PCR for 13 genes: ACTB, HPRT, vGLUT1, βTUBIII, COMT, DISC1, GAD1, PAX6, DTNBP1, ERBB4, FOXP1, FOXP2, and GIRK2. Neurons derived from both iPSC lines expressed βTUBIII, fired action potentials, and experienced spontaneous depolarizations (UP states) ~. 2. weeks before vGLUT1, GAD1 and GIRK2 appeared. Multisite calcium imaging revealed that these UP states were not synchronized among hESC-H9-derived neurons. The expression of FOXP1, FOXP2 and vGLUT1 was lost after 50. days in culture, in contrast to other continuously expressed genes. When gene expression was combined with electrophysiology, two subsets of genes were apparent; those irrelevant to spontaneous depolarizations (including vGLUT1, GIRK2, FOXP2 and DISC1) and those associated with spontaneous depolarizations (GAD1 and ERBB4). The results demonstrate that in the earliest stages of neuron development, it is useful to combine genetic analysis with physiological characterizations, on a cell-to-cell basis.

Original languageEnglish (US)
Pages (from-to)101-118
Number of pages18
JournalStem Cell Research
Volume12
Issue number1
DOIs
StatePublished - Jan 2014

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Calcium
Neurons
Polymerase Chain Reaction
Genes
Hypoxanthine Phosphoribosyltransferase
Electrophysiology
Action Potentials
Molecular Biology
Healthy Volunteers
Electrodes
Gene Expression

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Medicine(all)

Cite this

Patch-clamp recordings and calcium imaging followed by single-cell PCR reveal the developmental profile of 13 genes in iPSC-derived human neurons. / Belinsky, Glenn S.; Rich, Matthew T.; Sirois, Carissa L.; Short, Shaina M.; Pedrosa, Erika; Lachman, Herbert M.; Antic, Srdjan D.

In: Stem Cell Research, Vol. 12, No. 1, 01.2014, p. 101-118.

Research output: Contribution to journalArticle

Belinsky, Glenn S. ; Rich, Matthew T. ; Sirois, Carissa L. ; Short, Shaina M. ; Pedrosa, Erika ; Lachman, Herbert M. ; Antic, Srdjan D. / Patch-clamp recordings and calcium imaging followed by single-cell PCR reveal the developmental profile of 13 genes in iPSC-derived human neurons. In: Stem Cell Research. 2014 ; Vol. 12, No. 1. pp. 101-118.
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