Passive administration of monoclonal antibodies to Anthrolysin O prolong survival in mice lethally infected with Bacillus anthracis

Antonio Nakouzi, Johanna Rivera, Richard F. Rest, Arturo Casadevall

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Background. Bacillus anthracis has two major virulence factors: a tripartite toxin that produces lethal and edema toxins and a polyglutamic acid capsule. A recent report suggested that a toxin belonging to the cholesterol dependant cytolysin (CDC) family, anthrolysin O (ALO) was a new virulence factor for B. anthracis but subsequent studies have questioned its relevance in pathogenesis. In this study, we examined the immunogenicity of recombinant anthrolysin O (rALO) in mice. Results. BALB/c mice immunized with rALO and boosted after two weeks, produce sera with strong Ab responses with a predominance of IgG1 and IgG2a. Five hybridomas to rALO were recovered representing the IgM, IgG1, and IgG2b isotypes. Passive administration of 3 of the five monoclonal antibodies (mAbs) to rALO prior to infection with lethal intravenous (i.v.) B. anthracis Sterne strain infection in mice was associated with enhanced average survival and a greater likelihood of surviving infection. A combination of two mAbs to ALO was more effective than either mAb separately. One mAb (64F8) slowed the toxicity of rALO for J774.16 macrophage-like cells. Conclusion. Our results suggest that ALO contributes to the virulence of B. anthracis Sterne strain in this infection model and that Ab response to ALO may contribute to protection in certain circumstances.

Original languageEnglish (US)
Article number159
JournalBMC Microbiology
Volume8
DOIs
StatePublished - 2008

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Bacillus anthracis
Monoclonal Antibodies
Virulence Factors
Infection
Immunoglobulin G
Polyglutamic Acid
Perforin
Hybridomas
Capsules
Immunoglobulin M
Virulence
Edema
Macrophages
Cholesterol
Serum

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Passive administration of monoclonal antibodies to Anthrolysin O prolong survival in mice lethally infected with Bacillus anthracis. / Nakouzi, Antonio; Rivera, Johanna; Rest, Richard F.; Casadevall, Arturo.

In: BMC Microbiology, Vol. 8, 159, 2008.

Research output: Contribution to journalArticle

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