Partial purification and characterization of a colony-stimulating factor secreted by a T lymphocyte clone

Michael B. Prystowsky, J. M. Ely, M. F. Naujokas, E. Goldwasser, F. W. Fitch

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The mouse T lymphocyte clone L2 secretes a minimum of 10 lymphokine activities affecting at least 5 different target cells. Large amounts of colony-stimulating factor (CSF) (8.6 x 106 U/ml) can be obtained by stimulating L2 cells with concanavalin A. The major CSF activity secreted by L2 cells has been enriched to a specific activity of approximately 2-4 x 108 colonies/mg of protein using hydrophobic-interaction, gel-permeation, ion-exchange, and lectin-affinity chromatography. This preparation of CSF contains no detectable interleukin 2, interleukin 3, or interferon. The major L2-cell CSF induces granulocyte/macrophage colonies from bone marrow cells. This GM CSF has an apparent Mr of 22,000 as determined by gel-permeation chromatography. Treatment of L2-cell CSF with proteolytic enzymes abrogates biologic activity.

Original languageEnglish (US)
Pages (from-to)931-943
Number of pages13
JournalExperimental Hematology
Volume11
Issue number10
StatePublished - 1983
Externally publishedYes

Fingerprint

Colony-Stimulating Factors
Clone Cells
T-Lymphocytes
Granulocyte-Macrophage Colony-Stimulating Factor
Lymphokines
Interleukin-3
Ion Exchange
Concanavalin A
Hydrophobic and Hydrophilic Interactions
Affinity Chromatography
Lectins
Bone Marrow Cells
Interferons
Interleukin-2
Gel Chromatography
Peptide Hydrolases
Gels
Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Partial purification and characterization of a colony-stimulating factor secreted by a T lymphocyte clone. / Prystowsky, Michael B.; Ely, J. M.; Naujokas, M. F.; Goldwasser, E.; Fitch, F. W.

In: Experimental Hematology, Vol. 11, No. 10, 1983, p. 931-943.

Research output: Contribution to journalArticle

Prystowsky, Michael B. ; Ely, J. M. ; Naujokas, M. F. ; Goldwasser, E. ; Fitch, F. W. / Partial purification and characterization of a colony-stimulating factor secreted by a T lymphocyte clone. In: Experimental Hematology. 1983 ; Vol. 11, No. 10. pp. 931-943.
@article{00858f282a2e4b568b8c47cb5dbf17e0,
title = "Partial purification and characterization of a colony-stimulating factor secreted by a T lymphocyte clone",
abstract = "The mouse T lymphocyte clone L2 secretes a minimum of 10 lymphokine activities affecting at least 5 different target cells. Large amounts of colony-stimulating factor (CSF) (8.6 x 106 U/ml) can be obtained by stimulating L2 cells with concanavalin A. The major CSF activity secreted by L2 cells has been enriched to a specific activity of approximately 2-4 x 108 colonies/mg of protein using hydrophobic-interaction, gel-permeation, ion-exchange, and lectin-affinity chromatography. This preparation of CSF contains no detectable interleukin 2, interleukin 3, or interferon. The major L2-cell CSF induces granulocyte/macrophage colonies from bone marrow cells. This GM CSF has an apparent Mr of 22,000 as determined by gel-permeation chromatography. Treatment of L2-cell CSF with proteolytic enzymes abrogates biologic activity.",
author = "Prystowsky, {Michael B.} and Ely, {J. M.} and Naujokas, {M. F.} and E. Goldwasser and Fitch, {F. W.}",
year = "1983",
language = "English (US)",
volume = "11",
pages = "931--943",
journal = "Experimental Hematology",
issn = "0301-472X",
publisher = "Elsevier Inc.",
number = "10",

}

TY - JOUR

T1 - Partial purification and characterization of a colony-stimulating factor secreted by a T lymphocyte clone

AU - Prystowsky, Michael B.

AU - Ely, J. M.

AU - Naujokas, M. F.

AU - Goldwasser, E.

AU - Fitch, F. W.

PY - 1983

Y1 - 1983

N2 - The mouse T lymphocyte clone L2 secretes a minimum of 10 lymphokine activities affecting at least 5 different target cells. Large amounts of colony-stimulating factor (CSF) (8.6 x 106 U/ml) can be obtained by stimulating L2 cells with concanavalin A. The major CSF activity secreted by L2 cells has been enriched to a specific activity of approximately 2-4 x 108 colonies/mg of protein using hydrophobic-interaction, gel-permeation, ion-exchange, and lectin-affinity chromatography. This preparation of CSF contains no detectable interleukin 2, interleukin 3, or interferon. The major L2-cell CSF induces granulocyte/macrophage colonies from bone marrow cells. This GM CSF has an apparent Mr of 22,000 as determined by gel-permeation chromatography. Treatment of L2-cell CSF with proteolytic enzymes abrogates biologic activity.

AB - The mouse T lymphocyte clone L2 secretes a minimum of 10 lymphokine activities affecting at least 5 different target cells. Large amounts of colony-stimulating factor (CSF) (8.6 x 106 U/ml) can be obtained by stimulating L2 cells with concanavalin A. The major CSF activity secreted by L2 cells has been enriched to a specific activity of approximately 2-4 x 108 colonies/mg of protein using hydrophobic-interaction, gel-permeation, ion-exchange, and lectin-affinity chromatography. This preparation of CSF contains no detectable interleukin 2, interleukin 3, or interferon. The major L2-cell CSF induces granulocyte/macrophage colonies from bone marrow cells. This GM CSF has an apparent Mr of 22,000 as determined by gel-permeation chromatography. Treatment of L2-cell CSF with proteolytic enzymes abrogates biologic activity.

UR - http://www.scopus.com/inward/record.url?scp=0021033248&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021033248&partnerID=8YFLogxK

M3 - Article

C2 - 6363113

AN - SCOPUS:0021033248

VL - 11

SP - 931

EP - 943

JO - Experimental Hematology

JF - Experimental Hematology

SN - 0301-472X

IS - 10

ER -