Partial purification and characterization of a colony-stimulating factor secreted by a T lymphocyte clone

M. B. Prystowsky, J. M. Ely, M. F. Naujokas, E. Goldwasser, F. W. Fitch

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The mouse T lymphocyte clone L2 secretes a minimum of 10 lymphokine activities affecting at least 5 different target cells. Large amounts of colony-stimulating factor (CSF) (8.6 x 106 U/ml) can be obtained by stimulating L2 cells with concanavalin A. The major CSF activity secreted by L2 cells has been enriched to a specific activity of approximately 2-4 x 108 colonies/mg of protein using hydrophobic-interaction, gel-permeation, ion-exchange, and lectin-affinity chromatography. This preparation of CSF contains no detectable interleukin 2, interleukin 3, or interferon. The major L2-cell CSF induces granulocyte/macrophage colonies from bone marrow cells. This GM CSF has an apparent Mr of 22,000 as determined by gel-permeation chromatography. Treatment of L2-cell CSF with proteolytic enzymes abrogates biologic activity.

Original languageEnglish (US)
Pages (from-to)931-943
Number of pages13
JournalExperimental Hematology
Volume11
Issue number10
StatePublished - Dec 1 1983
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Hematology
  • Genetics
  • Cell Biology
  • Cancer Research

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