Partial primary structures of human and murine macrophage colony stimulating factor (CSF-1)

Albert Boosman, James E. Strickler, Kenneth J. Wilson, E. Richard Stanley

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Approximately 40 amino-terminal residues and 20 internal residues of CSF-1 purified from the media of cultured human pancreatic carcinoma (MIA PaCa) cells and of cultured murine L cells have been identified. Results indicated that the two subunits in each molecule of biologically active CSF-1 are identical in their amino-terminal portions. The twelve amino-terminal residues of MIA PaCa CSF-1 were found to be identical to those of human-urinary CSF-1, suggesting that the polypeptide portions of the two human proteins may be identical. Approximately 75% of the amino acids identified in both MIA PaCa CSF-1 and murine CSF-1 were found to be common to both. No homology to other proteins was observed. This study suggests a subunit polypeptide Mr nearer to 17K than to 26K predicted from cDNA.

Original languageEnglish (US)
Pages (from-to)74-80
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume144
Issue number1
DOIs
StatePublished - Apr 14 1987

Fingerprint

Macrophage Colony-Stimulating Factor
Peptides
Cultured Cells
Proteins
Complementary DNA
Cells
Amino Acids
Molecules
Pancreatic Carcinoma

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Partial primary structures of human and murine macrophage colony stimulating factor (CSF-1). / Boosman, Albert; Strickler, James E.; Wilson, Kenneth J.; Stanley, E. Richard.

In: Biochemical and Biophysical Research Communications, Vol. 144, No. 1, 14.04.1987, p. 74-80.

Research output: Contribution to journalArticle

Boosman, Albert ; Strickler, James E. ; Wilson, Kenneth J. ; Stanley, E. Richard. / Partial primary structures of human and murine macrophage colony stimulating factor (CSF-1). In: Biochemical and Biophysical Research Communications. 1987 ; Vol. 144, No. 1. pp. 74-80.
@article{c38da7299bfe40218067d9d18f0d907a,
title = "Partial primary structures of human and murine macrophage colony stimulating factor (CSF-1)",
abstract = "Approximately 40 amino-terminal residues and 20 internal residues of CSF-1 purified from the media of cultured human pancreatic carcinoma (MIA PaCa) cells and of cultured murine L cells have been identified. Results indicated that the two subunits in each molecule of biologically active CSF-1 are identical in their amino-terminal portions. The twelve amino-terminal residues of MIA PaCa CSF-1 were found to be identical to those of human-urinary CSF-1, suggesting that the polypeptide portions of the two human proteins may be identical. Approximately 75{\%} of the amino acids identified in both MIA PaCa CSF-1 and murine CSF-1 were found to be common to both. No homology to other proteins was observed. This study suggests a subunit polypeptide Mr nearer to 17K than to 26K predicted from cDNA.",
author = "Albert Boosman and Strickler, {James E.} and Wilson, {Kenneth J.} and Stanley, {E. Richard}",
year = "1987",
month = "4",
day = "14",
doi = "10.1016/S0006-291X(87)80477-1",
language = "English (US)",
volume = "144",
pages = "74--80",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Partial primary structures of human and murine macrophage colony stimulating factor (CSF-1)

AU - Boosman, Albert

AU - Strickler, James E.

AU - Wilson, Kenneth J.

AU - Stanley, E. Richard

PY - 1987/4/14

Y1 - 1987/4/14

N2 - Approximately 40 amino-terminal residues and 20 internal residues of CSF-1 purified from the media of cultured human pancreatic carcinoma (MIA PaCa) cells and of cultured murine L cells have been identified. Results indicated that the two subunits in each molecule of biologically active CSF-1 are identical in their amino-terminal portions. The twelve amino-terminal residues of MIA PaCa CSF-1 were found to be identical to those of human-urinary CSF-1, suggesting that the polypeptide portions of the two human proteins may be identical. Approximately 75% of the amino acids identified in both MIA PaCa CSF-1 and murine CSF-1 were found to be common to both. No homology to other proteins was observed. This study suggests a subunit polypeptide Mr nearer to 17K than to 26K predicted from cDNA.

AB - Approximately 40 amino-terminal residues and 20 internal residues of CSF-1 purified from the media of cultured human pancreatic carcinoma (MIA PaCa) cells and of cultured murine L cells have been identified. Results indicated that the two subunits in each molecule of biologically active CSF-1 are identical in their amino-terminal portions. The twelve amino-terminal residues of MIA PaCa CSF-1 were found to be identical to those of human-urinary CSF-1, suggesting that the polypeptide portions of the two human proteins may be identical. Approximately 75% of the amino acids identified in both MIA PaCa CSF-1 and murine CSF-1 were found to be common to both. No homology to other proteins was observed. This study suggests a subunit polypeptide Mr nearer to 17K than to 26K predicted from cDNA.

UR - http://www.scopus.com/inward/record.url?scp=0023216254&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023216254&partnerID=8YFLogxK

U2 - 10.1016/S0006-291X(87)80477-1

DO - 10.1016/S0006-291X(87)80477-1

M3 - Article

C2 - 3495265

AN - SCOPUS:0023216254

VL - 144

SP - 74

EP - 80

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -