Partial primary structures of human and murine macrophage colony stimulating factor (CSF-1)

Albert Boosman, James E. Strickler, Kenneth J. Wilson, E. Richard Stanley

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Approximately 40 amino-terminal residues and 20 internal residues of CSF-1 purified from the media of cultured human pancreatic carcinoma (MIA PaCa) cells and of cultured murine L cells have been identified. Results indicated that the two subunits in each molecule of biologically active CSF-1 are identical in their amino-terminal portions. The twelve amino-terminal residues of MIA PaCa CSF-1 were found to be identical to those of human-urinary CSF-1, suggesting that the polypeptide portions of the two human proteins may be identical. Approximately 75% of the amino acids identified in both MIA PaCa CSF-1 and murine CSF-1 were found to be common to both. No homology to other proteins was observed. This study suggests a subunit polypeptide Mr nearer to 17K than to 26K predicted from cDNA.

Original languageEnglish (US)
Pages (from-to)74-80
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume144
Issue number1
DOIs
Publication statusPublished - Apr 14 1987

    Fingerprint

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this