Partial hepatectomy and subsequent radiation facilitates engraftment of mouse embryonic stem cells in the liver

N. Kobayashi, M. Ando, Y. Kosaka, C. Yong, T. Okitsu, T. Arata, H. Ikeda, K. Kobayashi, T. Ueda, Y. Kurabayashi, N. Tanaka

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6 Scopus citations

Abstract

For liver-targeted regenerative medicine, embryonic stem (ES) cell-derived hepatocyte-like cells proffer great expectation. In vitro exposure to a combination of various growth factors, such as hepatocyte growth factor and fibroblast growth factor-4, as well as cytokines, leads to differentiation of ES cells into hepatocyte-like cells. We sought to determine the in vivo environment that allowed engraftment of ES cells transplanted to the liver. Thus, we examined the effect of partial hepatectomy (50%) (PHT) and subsequent radiation (RT) of the male Balb/c mouse host liver on ES cell engraftment. ES cells (5 × 10 6) derived from 129Sv mice were transplanted into the residual liver. The controls were ES cells transplanted into a normal liver. Bromo-deoxy-residine (BrdU)-uptake was performed to evaluate the effect of hepatectomy and RT on hepatocyte regeneration. Mouse ES cells engrafted, forming teratomas in the normal liver without showing any mononuclear infiltration. A liver modified by PHT and RT facilitated engraftment of mouse ES cells compared with a normal liver. Hepatic RT significantly suppressed hepatocytic uptake of BrdU.

Original languageEnglish (US)
Pages (from-to)2352-2354
Number of pages3
JournalTransplantation Proceedings
Volume36
Issue number8
DOIs
Publication statusPublished - Oct 1 2004

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ASJC Scopus subject areas

  • Surgery
  • Transplantation

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