Partial excision of bla KPC from Tn4401 in carbapenem-resistant Klebsiella pneumoniae

Liang Chen, Kalyan D. Chavda, José R. Mediavilla, Michael R. Jacobs, Michael H. Levi, Robert A. Bonomo, Barry N. Kreiswirth

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We describe a novel Tn4401 variant (Tn4401d) in epidemic Klebsiella pneumoniae clone ST258, from which a partial bla KPCfragment has been excised along with ISKpn7 and a partial tnpA fragment. Nested-PCR experiments confirmed that this region can be removed from distinct Tn4401 isoforms in both K. pneumoniae and Escherichia coli. This study highlights that the region surrounding bla KPC is undergoing recombination and that Tn4401 itself is heterogeneous and highly plastic.

Original languageEnglish (US)
Pages (from-to)1635-1638
Number of pages4
JournalAntimicrobial Agents and Chemotherapy
Volume56
Issue number3
DOIs
StatePublished - Mar 2012

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Carbapenems
Klebsiella pneumoniae
Genetic Recombination
Plastics
Protein Isoforms
Clone Cells
Escherichia coli
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Pharmacology
  • Infectious Diseases

Cite this

Chen, L., Chavda, K. D., Mediavilla, J. R., Jacobs, M. R., Levi, M. H., Bonomo, R. A., & Kreiswirth, B. N. (2012). Partial excision of bla KPC from Tn4401 in carbapenem-resistant Klebsiella pneumoniae. Antimicrobial Agents and Chemotherapy, 56(3), 1635-1638. https://doi.org/10.1128/AAC.06182-11

Partial excision of bla KPC from Tn4401 in carbapenem-resistant Klebsiella pneumoniae. / Chen, Liang; Chavda, Kalyan D.; Mediavilla, José R.; Jacobs, Michael R.; Levi, Michael H.; Bonomo, Robert A.; Kreiswirth, Barry N.

In: Antimicrobial Agents and Chemotherapy, Vol. 56, No. 3, 03.2012, p. 1635-1638.

Research output: Contribution to journalArticle

Chen, L, Chavda, KD, Mediavilla, JR, Jacobs, MR, Levi, MH, Bonomo, RA & Kreiswirth, BN 2012, 'Partial excision of bla KPC from Tn4401 in carbapenem-resistant Klebsiella pneumoniae', Antimicrobial Agents and Chemotherapy, vol. 56, no. 3, pp. 1635-1638. https://doi.org/10.1128/AAC.06182-11
Chen, Liang ; Chavda, Kalyan D. ; Mediavilla, José R. ; Jacobs, Michael R. ; Levi, Michael H. ; Bonomo, Robert A. ; Kreiswirth, Barry N. / Partial excision of bla KPC from Tn4401 in carbapenem-resistant Klebsiella pneumoniae. In: Antimicrobial Agents and Chemotherapy. 2012 ; Vol. 56, No. 3. pp. 1635-1638.
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