Partial characterization of a fibroblast-stimulating factor produced by cloned murine T lymphocytes

P. J. Lammie, J. G. Monroe, A. I. Michael, G. D. Johnson, S. M. Phillips, M. B. Prystowsky

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7 Scopus citations

Abstract

T cells may regulate tissue fibrosis through the elaboration of soluble factors that stimulate fibroblast growth. The authors previously identified a factor produced by cloned Schistosoma mansoni antigen-specific T cells which served as a competence factor for murine fibroblasts. In the present report, they further characterize this fibroblast-stimulating factor (FsF) and differentiate it from a number of other T-cell-derived lymphokine activities. Crude supernatants from concanavalin-activated cloned T cells were fractionated by gel filtration, ion exchange, or reversed-phase high-pressure liquid chromatography. FsF has an apparent molecular weight of 17,000 and could be differentiated from colony-stimulating factor (CSF), interleukin-3 (IL-3), and interferon (IFN) on the basis of chromatographic characteristics. Highly purified or recombinant IL-2, IL-3, CSF, and IFN had no significant effect on fibroblast proliferation. Furthermore, a monoclonal anti-B-cell-stimulating factor-1 antibody only partially blocked the fibroblast proliferation induced by T-cell supernatants.

Original languageEnglish (US)
Pages (from-to)289-295
Number of pages7
JournalAmerican Journal of Pathology
Volume130
Issue number2
StatePublished - Jan 1 1988
Externally publishedYes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    Lammie, P. J., Monroe, J. G., Michael, A. I., Johnson, G. D., Phillips, S. M., & Prystowsky, M. B. (1988). Partial characterization of a fibroblast-stimulating factor produced by cloned murine T lymphocytes. American Journal of Pathology, 130(2), 289-295.