Partial characterization of a fibroblast-stimulating factor produced by cloned murine T lymphocytes

P. J. Lammie, J. G. Monroe, A. I. Michael, G. D. Johnson, S. M. Phillips, Michael B. Prystowsky

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

T cells may regulate tissue fibrosis through the elaboration of soluble factors that stimulate fibroblast growth. The authors previously identified a factor produced by cloned Schistosoma mansoni antigen-specific T cells which served as a competence factor for murine fibroblasts. In the present report, they further characterize this fibroblast-stimulating factor (FsF) and differentiate it from a number of other T-cell-derived lymphokine activities. Crude supernatants from concanavalin-activated cloned T cells were fractionated by gel filtration, ion exchange, or reversed-phase high-pressure liquid chromatography. FsF has an apparent molecular weight of 17,000 and could be differentiated from colony-stimulating factor (CSF), interleukin-3 (IL-3), and interferon (IFN) on the basis of chromatographic characteristics. Highly purified or recombinant IL-2, IL-3, CSF, and IFN had no significant effect on fibroblast proliferation. Furthermore, a monoclonal anti-B-cell-stimulating factor-1 antibody only partially blocked the fibroblast proliferation induced by T-cell supernatants.

Original languageEnglish (US)
Pages (from-to)289-295
Number of pages7
JournalAmerican Journal of Pathology
Volume130
Issue number2
StatePublished - 1988
Externally publishedYes

Fingerprint

Fibroblasts
T-Lymphocytes
Colony-Stimulating Factors
Interleukin-3
Interferons
Fibroblast Growth Factors
Schistosoma mansoni
Lymphokines
Ion Exchange
Reverse-Phase Chromatography
Interleukin-4
Mental Competency
Interleukin-2
Gel Chromatography
Fibrosis
Molecular Weight
High Pressure Liquid Chromatography
Antigens
Antibodies

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Lammie, P. J., Monroe, J. G., Michael, A. I., Johnson, G. D., Phillips, S. M., & Prystowsky, M. B. (1988). Partial characterization of a fibroblast-stimulating factor produced by cloned murine T lymphocytes. American Journal of Pathology, 130(2), 289-295.

Partial characterization of a fibroblast-stimulating factor produced by cloned murine T lymphocytes. / Lammie, P. J.; Monroe, J. G.; Michael, A. I.; Johnson, G. D.; Phillips, S. M.; Prystowsky, Michael B.

In: American Journal of Pathology, Vol. 130, No. 2, 1988, p. 289-295.

Research output: Contribution to journalArticle

Lammie, PJ, Monroe, JG, Michael, AI, Johnson, GD, Phillips, SM & Prystowsky, MB 1988, 'Partial characterization of a fibroblast-stimulating factor produced by cloned murine T lymphocytes', American Journal of Pathology, vol. 130, no. 2, pp. 289-295.
Lammie, P. J. ; Monroe, J. G. ; Michael, A. I. ; Johnson, G. D. ; Phillips, S. M. ; Prystowsky, Michael B. / Partial characterization of a fibroblast-stimulating factor produced by cloned murine T lymphocytes. In: American Journal of Pathology. 1988 ; Vol. 130, No. 2. pp. 289-295.
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