Parathyroid mitogenic activity in plasma from patients with familial multiple endocrine neoplasia type 1

M. L. Brandi, G. D. Aurbach, L. A. Fitzpatrick, R. Quarto, Allen M. Spiegel, M. M. Bliziotes, J. A. Norton, J. L. Doppman, S. J. Marx

Research output: Contribution to journalArticle

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Abstract

Hyperplasia of the parathyroid glands is a central feature of familial multiple endocrine neoplasia type 1. We used cultured bovine parathyroid cells to test for mitogenic activity in plasma from patients with this disorder. Normal plasma stimulated [ 3H]thymidine incorporation, on the average, to the same extent as it was stimulated in a plasma-free control culture. This contrasted with the results of the tests with plasma from patients with familial multiple endocrine neoplasia type 1, in which parathyroid mitogenic activity increased 2400 percent over the control value (P < 0.001). Plasma from these patients also stimulated the proliferation of bovine parathyroid cells in culture, whereas plasma from normal subjects inhibited it. Parathyroid mitogenic activity in plasma from the patients with familial multiple endocrine neoplasia type 1 was greater than that in plasma from patients with various other disorders, including sporadic primary hyperparathyroidism (with adenoma, hyperplasia, or cancer or the parathyroid), sporadic primary hypergastrinemia, sporadic pituitary tumor, familial hypocalciuric hypercalcemia, and multiple endocrine neoplasia type 2 (P < 0.05). Parathyroid mitogenic activity in the plasma of patients with familial multiple endocrine neoplasia type 1 persisted for up to four years after total parathyroidectomy. The plasma also had far more mitogenic activity in cultures of parathyroid cells than did optimal concentrations of known growth factors or of any parathyroid secretagogue. This mitogenic activity had an apparent molecular weight of 50,000 to 55,000. We conclude that primary hyperparathyroidism in familial multiple endocrine neoplasia type 1 may have a humoral cause.

Original languageEnglish (US)
Pages (from-to)1287-1293
Number of pages7
JournalNew England Journal of Medicine
Volume314
Issue number20
StatePublished - 1986
Externally publishedYes

Fingerprint

Multiple Endocrine Neoplasia Type 1
Primary Hyperparathyroidism
Hyperplasia
Cell Culture Techniques
Multiple Endocrine Neoplasia Type 2a
Parathyroid Neoplasms
Parathyroidectomy
Parathyroid Glands
Pituitary Neoplasms
Adenoma
Thymidine
Intercellular Signaling Peptides and Proteins
Molecular Weight

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Brandi, M. L., Aurbach, G. D., Fitzpatrick, L. A., Quarto, R., Spiegel, A. M., Bliziotes, M. M., ... Marx, S. J. (1986). Parathyroid mitogenic activity in plasma from patients with familial multiple endocrine neoplasia type 1. New England Journal of Medicine, 314(20), 1287-1293.

Parathyroid mitogenic activity in plasma from patients with familial multiple endocrine neoplasia type 1. / Brandi, M. L.; Aurbach, G. D.; Fitzpatrick, L. A.; Quarto, R.; Spiegel, Allen M.; Bliziotes, M. M.; Norton, J. A.; Doppman, J. L.; Marx, S. J.

In: New England Journal of Medicine, Vol. 314, No. 20, 1986, p. 1287-1293.

Research output: Contribution to journalArticle

Brandi, ML, Aurbach, GD, Fitzpatrick, LA, Quarto, R, Spiegel, AM, Bliziotes, MM, Norton, JA, Doppman, JL & Marx, SJ 1986, 'Parathyroid mitogenic activity in plasma from patients with familial multiple endocrine neoplasia type 1', New England Journal of Medicine, vol. 314, no. 20, pp. 1287-1293.
Brandi, M. L. ; Aurbach, G. D. ; Fitzpatrick, L. A. ; Quarto, R. ; Spiegel, Allen M. ; Bliziotes, M. M. ; Norton, J. A. ; Doppman, J. L. ; Marx, S. J. / Parathyroid mitogenic activity in plasma from patients with familial multiple endocrine neoplasia type 1. In: New England Journal of Medicine. 1986 ; Vol. 314, No. 20. pp. 1287-1293.
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