Paraquat toxicity and pyridine nucleotide coenzyme synthesis: A data correction

Olen R. Brown, Richard Seither

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The decrease in pyridine nucleotide coenzymes which occurs during poisoning of Escherichia coli by hyperbaric oxygen or paraquat is not due to impairment of nicotinatemononucleotide pyrophosphorylase (carboxylating) [EC 2.4.2.19] as was previously proposed (Brown, O.R. et al. Biochem. Biophys. Res. Commun. 91:982-990; 1979). This was shown directly using extracts of E. coli, prepared after exposure to 1 mM paraquat or 4.2 atmospheres of oxygen. The enzyme also was not impaired in Neurospora crassa by 1 mM paraquat. A naturally-occurring, non-dialyzable inhibitor of the enzyme was found in E. coli extracts. The inhibitor caused the erroneous, low nicotinatemononucleotide pyrophosphorylase (carboxylating) activities previously reported in extracts of E. coli poisoned by paraquat.

Original languageEnglish (US)
Pages (from-to)113-116
Number of pages4
JournalFree Radical Biology and Medicine
Volume8
Issue number2
DOIs
StatePublished - 1990
Externally publishedYes

Fingerprint

Paraquat
Coenzymes
Escherichia coli
Toxicity
Nucleotides
Oxygen
Neurospora crassa
Enzyme Inhibitors
Atmosphere
Poisoning
pyridine
Enzymes
nicotinate-nucleotide diphosphorylase (carboxylating)

Keywords

  • Free radicals
  • Nicotinatemononucleotide pyrophosphorylase (carboxylating)
  • Oxidant stress
  • Oxygen toxicity
  • Paraquat toxicity
  • Pyridine nucleotide coenzymes
  • Superoxide

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Medicine(all)
  • Toxicology

Cite this

Paraquat toxicity and pyridine nucleotide coenzyme synthesis : A data correction. / Brown, Olen R.; Seither, Richard.

In: Free Radical Biology and Medicine, Vol. 8, No. 2, 1990, p. 113-116.

Research output: Contribution to journalArticle

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