Paramecium fusion rosettes: Possible function as Ca2+ gates

Birgit H. Satir, Stephen G. Oberg

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

The function of a specific intramembrane particle array, "the fusion rosette," an essential requirement for exocytosis of trichocysts in Paramecium, was probed with a temperature sensitive secretory mutant (nd9). The cells were grown at 27°C, the nonpermissive, nonreleasing temperature at which fusion rosettes do not assemble. Exocytosis could be triggered, nonetheless, by addition of 40 μM ionophore A23187 and 15 mM Ca2+ but not Mg2+. Rosette function is bypassed by this procedure, suggesting that during normal release, the rosette acts as a Ca2+ channel that allows development of a site-specific increase in Ca2+, which in turn induces fusion and release.

Original languageEnglish (US)
Pages (from-to)536-538
Number of pages3
JournalScience
Volume199
Issue number4328
DOIs
StatePublished - Jan 1 1978

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Paramecium fusion rosettes: Possible function as Ca<sup>2+</sup> gates'. Together they form a unique fingerprint.

  • Cite this