Paramecium fusion rosettes: Possible function as Ca2+ gates

Birgit H. Satir, Stephen G. Oberg

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The function of a specific intramembrane particle array, "the fusion rosette," an essential requirement for exocytosis of trichocysts in Paramecium, was probed with a temperature sensitive secretory mutant (nd9). The cells were grown at 27°C, the nonpermissive, nonreleasing temperature at which fusion rosettes do not assemble. Exocytosis could be triggered, nonetheless, by addition of 40 μM ionophore A23187 and 15 mM Ca2+ but not Mg2+. Rosette function is bypassed by this procedure, suggesting that during normal release, the rosette acts as a Ca2+ channel that allows development of a site-specific increase in Ca2+, which in turn induces fusion and release.

Original languageEnglish (US)
Pages (from-to)536-538
Number of pages3
JournalScience
Volume199
Issue number4328
StatePublished - 1978
Externally publishedYes

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Paramecium
Exocytosis
Temperature
Ionophores
Calcimycin

ASJC Scopus subject areas

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Cite this

Satir, B. H., & Oberg, S. G. (1978). Paramecium fusion rosettes: Possible function as Ca2+ gates. Science, 199(4328), 536-538.

Paramecium fusion rosettes : Possible function as Ca2+ gates. / Satir, Birgit H.; Oberg, Stephen G.

In: Science, Vol. 199, No. 4328, 1978, p. 536-538.

Research output: Contribution to journalArticle

Satir, BH & Oberg, SG 1978, 'Paramecium fusion rosettes: Possible function as Ca2+ gates', Science, vol. 199, no. 4328, pp. 536-538.
Satir BH, Oberg SG. Paramecium fusion rosettes: Possible function as Ca2+ gates. Science. 1978;199(4328):536-538.
Satir, Birgit H. ; Oberg, Stephen G. / Paramecium fusion rosettes : Possible function as Ca2+ gates. In: Science. 1978 ; Vol. 199, No. 4328. pp. 536-538.
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