Paracrine stimulation of endothelial cell motility and angiogenesis by platelet-derived deoxyribose-1-phosphate

Giordano Pula, Elena Garonna, Warwick B. Dunn, Michio Hirano, Giuseppe Pizzorno, Michelangelo Campanella, Edward L. Schwartz, Mahmoud H. El Kouni, Caroline P D Wheeler-Jones

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Objective- Micromolar concentrations of the proangiogenic metabolite deoxyribose-1-phosphate (dRP) were detected in platelet supernatants by mass spectrometry. In this study, we assessed whether the release of dRP by platelets stimulates endothelial cell migration and angiogenesis. Methods and Results- Protein-free supernatants from thrombin-stimulated platelets increased human umbilical vein endothelial cell migratory activity in transmigration and monolayer repair assays. This phenomenon was ablated by genetic silencing of dRP-generating uridine phosphorylase (UP) and thymidine phosphorylase (TP) or pharmacological inhibition of UP and restored by exogenous dRP. The stimulation of endothelial cell migration by platelet-derived dRP correlated with upregulation of integrin β3, which was induced in a reactive oxygen species-dependent manner, and was mediated by the activity of the integrin heterodimer αvβ3. The physiological relevance of dRP release by platelets was confirmed in a chick chorioallantoic membrane assay, where the presence of this metabolite in platelet supernatants strongly induced capillary formation. Conclusion- Platelet-derived dRP stimulates endothelial cell migration by upregulating integrin β3 in a reactive oxygen species-dependent manner. As demonstrated by our in vivo experiments, this novel paracrine regulatory pathway is likely to play an important role in the stimulation of angiogenesis by platelets.

Original languageEnglish (US)
Pages (from-to)2631-2638
Number of pages8
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume30
Issue number12
DOIs
StatePublished - Dec 2010

Fingerprint

Deoxyribose
Cell Movement
Blood Platelets
Endothelial Cells
Phosphates
Uridine Phosphorylase
Integrins
Reactive Oxygen Species
Thymidine Phosphorylase
Chorioallantoic Membrane
Human Umbilical Vein Endothelial Cells
Thrombin
Mass Spectrometry
Up-Regulation
Pharmacology

Keywords

  • angiogenesis
  • deoxyribose-1-phosphate
  • endothelium
  • platelets
  • reactive oxygen species

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Pula, G., Garonna, E., Dunn, W. B., Hirano, M., Pizzorno, G., Campanella, M., ... Wheeler-Jones, C. P. D. (2010). Paracrine stimulation of endothelial cell motility and angiogenesis by platelet-derived deoxyribose-1-phosphate. Arteriosclerosis, Thrombosis, and Vascular Biology, 30(12), 2631-2638. https://doi.org/10.1161/ATVBAHA.110.215855

Paracrine stimulation of endothelial cell motility and angiogenesis by platelet-derived deoxyribose-1-phosphate. / Pula, Giordano; Garonna, Elena; Dunn, Warwick B.; Hirano, Michio; Pizzorno, Giuseppe; Campanella, Michelangelo; Schwartz, Edward L.; El Kouni, Mahmoud H.; Wheeler-Jones, Caroline P D.

In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 30, No. 12, 12.2010, p. 2631-2638.

Research output: Contribution to journalArticle

Pula, G, Garonna, E, Dunn, WB, Hirano, M, Pizzorno, G, Campanella, M, Schwartz, EL, El Kouni, MH & Wheeler-Jones, CPD 2010, 'Paracrine stimulation of endothelial cell motility and angiogenesis by platelet-derived deoxyribose-1-phosphate', Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 30, no. 12, pp. 2631-2638. https://doi.org/10.1161/ATVBAHA.110.215855
Pula, Giordano ; Garonna, Elena ; Dunn, Warwick B. ; Hirano, Michio ; Pizzorno, Giuseppe ; Campanella, Michelangelo ; Schwartz, Edward L. ; El Kouni, Mahmoud H. ; Wheeler-Jones, Caroline P D. / Paracrine stimulation of endothelial cell motility and angiogenesis by platelet-derived deoxyribose-1-phosphate. In: Arteriosclerosis, Thrombosis, and Vascular Biology. 2010 ; Vol. 30, No. 12. pp. 2631-2638.
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