Paracrine signaling through plasma membrane hemichannels

Nan Wang, Marijke De Bock, Elke Decrock, Mélissa Bol, Ashish Gadicherla, Mathieu Vinken, Vera Rogiers, Feliksas F. Bukauskas, Geert Bultynck, Luc Leybaert

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

Plasma membrane hemichannels composed of connexin (Cx) proteins are essential components of gap junction channels but accumulating evidence suggests functions of hemichannels beyond the communication provided by junctional channels. Hemichannels not incorporated into gap junctions, called unapposed hemichannels, can open in response to a variety of signals, electrical and chemical, thereby forming a conduit between the cell's interior and the extracellular milieu. Open hemichannels allow the bidirectional passage of ions and small metabolic or signaling molecules of below 1-2 kDa molecular weight. In addition to connexins, hemichannels can also be formed by pannexin (Panx) proteins and current evidence suggests that Cx26, Cx32, Cx36, Cx43 and Panx1, form hemichannels that allow the diffusive release of paracrine messengers. In particular, the case is strong for ATP but substantial evidence is also available for other messengers like glutamate and prostaglandins or metabolic substances like NAD+ or glutathione. While this field is clearly in expansion, evidence is still lacking at essential points of the paracrine signaling cascade that includes not only messenger release, but also downstream receptor signaling and consequent functional effects. The data available at this moment largely derives from in vitro experiments and still suffers from the difficulty of separating the functions of connexin-based hemichannels from gap junctions and from pannexin hemichannels. However, messengers like ATP or glutamate have universal roles in the body and further defining the contribution of hemichannels as a possible release pathway is expected to open novel avenues for better understanding their contribution to a variety of physiological and pathological processes. This article is part of a Special Issue entitled: The Communicating junctions, roles and dysfunctions.

Original languageEnglish (US)
Pages (from-to)35-50
Number of pages16
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1828
Issue number1
DOIs
StatePublished - Jan 2013

Fingerprint

Paracrine Communication
Connexins
Gap Junctions
Cell membranes
Cell Membrane
Glutamic Acid
Adenosine Triphosphate
Physiological Phenomena
Connexin 43
Pathologic Processes
NAD
Prostaglandins
Glutathione
Proteins
Molecular Weight
Molecular weight
Ions
Molecules
Communication
Experiments

Keywords

  • Connexin hemichannel
  • Pannexin hemichannel
  • Paracrine signaling

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biophysics

Cite this

Wang, N., De Bock, M., Decrock, E., Bol, M., Gadicherla, A., Vinken, M., ... Leybaert, L. (2013). Paracrine signaling through plasma membrane hemichannels. Biochimica et Biophysica Acta - Biomembranes, 1828(1), 35-50. https://doi.org/10.1016/j.bbamem.2012.07.002

Paracrine signaling through plasma membrane hemichannels. / Wang, Nan; De Bock, Marijke; Decrock, Elke; Bol, Mélissa; Gadicherla, Ashish; Vinken, Mathieu; Rogiers, Vera; Bukauskas, Feliksas F.; Bultynck, Geert; Leybaert, Luc.

In: Biochimica et Biophysica Acta - Biomembranes, Vol. 1828, No. 1, 01.2013, p. 35-50.

Research output: Contribution to journalArticle

Wang, N, De Bock, M, Decrock, E, Bol, M, Gadicherla, A, Vinken, M, Rogiers, V, Bukauskas, FF, Bultynck, G & Leybaert, L 2013, 'Paracrine signaling through plasma membrane hemichannels', Biochimica et Biophysica Acta - Biomembranes, vol. 1828, no. 1, pp. 35-50. https://doi.org/10.1016/j.bbamem.2012.07.002
Wang, Nan ; De Bock, Marijke ; Decrock, Elke ; Bol, Mélissa ; Gadicherla, Ashish ; Vinken, Mathieu ; Rogiers, Vera ; Bukauskas, Feliksas F. ; Bultynck, Geert ; Leybaert, Luc. / Paracrine signaling through plasma membrane hemichannels. In: Biochimica et Biophysica Acta - Biomembranes. 2013 ; Vol. 1828, No. 1. pp. 35-50.
@article{e583cc142fbb4320a4c4bc7c4fa461a9,
title = "Paracrine signaling through plasma membrane hemichannels",
abstract = "Plasma membrane hemichannels composed of connexin (Cx) proteins are essential components of gap junction channels but accumulating evidence suggests functions of hemichannels beyond the communication provided by junctional channels. Hemichannels not incorporated into gap junctions, called unapposed hemichannels, can open in response to a variety of signals, electrical and chemical, thereby forming a conduit between the cell's interior and the extracellular milieu. Open hemichannels allow the bidirectional passage of ions and small metabolic or signaling molecules of below 1-2 kDa molecular weight. In addition to connexins, hemichannels can also be formed by pannexin (Panx) proteins and current evidence suggests that Cx26, Cx32, Cx36, Cx43 and Panx1, form hemichannels that allow the diffusive release of paracrine messengers. In particular, the case is strong for ATP but substantial evidence is also available for other messengers like glutamate and prostaglandins or metabolic substances like NAD+ or glutathione. While this field is clearly in expansion, evidence is still lacking at essential points of the paracrine signaling cascade that includes not only messenger release, but also downstream receptor signaling and consequent functional effects. The data available at this moment largely derives from in vitro experiments and still suffers from the difficulty of separating the functions of connexin-based hemichannels from gap junctions and from pannexin hemichannels. However, messengers like ATP or glutamate have universal roles in the body and further defining the contribution of hemichannels as a possible release pathway is expected to open novel avenues for better understanding their contribution to a variety of physiological and pathological processes. This article is part of a Special Issue entitled: The Communicating junctions, roles and dysfunctions.",
keywords = "Connexin hemichannel, Pannexin hemichannel, Paracrine signaling",
author = "Nan Wang and {De Bock}, Marijke and Elke Decrock and M{\'e}lissa Bol and Ashish Gadicherla and Mathieu Vinken and Vera Rogiers and Bukauskas, {Feliksas F.} and Geert Bultynck and Luc Leybaert",
year = "2013",
month = "1",
doi = "10.1016/j.bbamem.2012.07.002",
language = "English (US)",
volume = "1828",
pages = "35--50",
journal = "Biochimica et Biophysica Acta - Biomembranes",
issn = "0005-2736",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Paracrine signaling through plasma membrane hemichannels

AU - Wang, Nan

AU - De Bock, Marijke

AU - Decrock, Elke

AU - Bol, Mélissa

AU - Gadicherla, Ashish

AU - Vinken, Mathieu

AU - Rogiers, Vera

AU - Bukauskas, Feliksas F.

AU - Bultynck, Geert

AU - Leybaert, Luc

PY - 2013/1

Y1 - 2013/1

N2 - Plasma membrane hemichannels composed of connexin (Cx) proteins are essential components of gap junction channels but accumulating evidence suggests functions of hemichannels beyond the communication provided by junctional channels. Hemichannels not incorporated into gap junctions, called unapposed hemichannels, can open in response to a variety of signals, electrical and chemical, thereby forming a conduit between the cell's interior and the extracellular milieu. Open hemichannels allow the bidirectional passage of ions and small metabolic or signaling molecules of below 1-2 kDa molecular weight. In addition to connexins, hemichannels can also be formed by pannexin (Panx) proteins and current evidence suggests that Cx26, Cx32, Cx36, Cx43 and Panx1, form hemichannels that allow the diffusive release of paracrine messengers. In particular, the case is strong for ATP but substantial evidence is also available for other messengers like glutamate and prostaglandins or metabolic substances like NAD+ or glutathione. While this field is clearly in expansion, evidence is still lacking at essential points of the paracrine signaling cascade that includes not only messenger release, but also downstream receptor signaling and consequent functional effects. The data available at this moment largely derives from in vitro experiments and still suffers from the difficulty of separating the functions of connexin-based hemichannels from gap junctions and from pannexin hemichannels. However, messengers like ATP or glutamate have universal roles in the body and further defining the contribution of hemichannels as a possible release pathway is expected to open novel avenues for better understanding their contribution to a variety of physiological and pathological processes. This article is part of a Special Issue entitled: The Communicating junctions, roles and dysfunctions.

AB - Plasma membrane hemichannels composed of connexin (Cx) proteins are essential components of gap junction channels but accumulating evidence suggests functions of hemichannels beyond the communication provided by junctional channels. Hemichannels not incorporated into gap junctions, called unapposed hemichannels, can open in response to a variety of signals, electrical and chemical, thereby forming a conduit between the cell's interior and the extracellular milieu. Open hemichannels allow the bidirectional passage of ions and small metabolic or signaling molecules of below 1-2 kDa molecular weight. In addition to connexins, hemichannels can also be formed by pannexin (Panx) proteins and current evidence suggests that Cx26, Cx32, Cx36, Cx43 and Panx1, form hemichannels that allow the diffusive release of paracrine messengers. In particular, the case is strong for ATP but substantial evidence is also available for other messengers like glutamate and prostaglandins or metabolic substances like NAD+ or glutathione. While this field is clearly in expansion, evidence is still lacking at essential points of the paracrine signaling cascade that includes not only messenger release, but also downstream receptor signaling and consequent functional effects. The data available at this moment largely derives from in vitro experiments and still suffers from the difficulty of separating the functions of connexin-based hemichannels from gap junctions and from pannexin hemichannels. However, messengers like ATP or glutamate have universal roles in the body and further defining the contribution of hemichannels as a possible release pathway is expected to open novel avenues for better understanding their contribution to a variety of physiological and pathological processes. This article is part of a Special Issue entitled: The Communicating junctions, roles and dysfunctions.

KW - Connexin hemichannel

KW - Pannexin hemichannel

KW - Paracrine signaling

UR - http://www.scopus.com/inward/record.url?scp=84870062221&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84870062221&partnerID=8YFLogxK

U2 - 10.1016/j.bbamem.2012.07.002

DO - 10.1016/j.bbamem.2012.07.002

M3 - Article

C2 - 22796188

AN - SCOPUS:84870062221

VL - 1828

SP - 35

EP - 50

JO - Biochimica et Biophysica Acta - Biomembranes

JF - Biochimica et Biophysica Acta - Biomembranes

SN - 0005-2736

IS - 1

ER -