Papillomavirus-associated focal oral hyperplasia in wild and captive asian lions (Panthera leo persica)

John P. Sundberg, Richard J. Montali, Mitchell Bush, Lyndsay G. Phillips, Stephen J. O'Brien, A. Bennett Jenson, Robert D. Burk, Marc Van Ranst

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Four Asian lions (Panthera leo persica), two wild and two captive, were diagnosed with focal oral hyperplasia affecting the ventral surface of their tongues. Focal, flat, sessile lesions consisted of hyperplastic, stratified squamous epithelium. Koilocytotic atypia was evident in the upper layers of cells, some of which contained characteristic intranuclear papillomavirus particles visible by electron microscopy. In addition, large amphophilic cytoplasmic inclusions were evident in the koilocytes and were considered to be a product of the viral E4 gene. Papillomavirus group-specific antigens were detected by immunohistochemistry in the atypical cell nuclei. Conserved papillomavirus antigenic epitopes differed from epitopes found in cutaneous papillomavirus-induced lesions from domestic cats. An 8,000-base pair DNA fragment, linearized by Bam HI digestion, was detected by Southern blot hybridization probed with a mixed human papillomavirus genomic probe. Limited restriction endonuclease studies of DNA prepared using an oral hyperplastic lesion from an Asian lion indicate that this is a novel feline papillomavirus different from the domestic cat cutaneous papillomavirus. This new virus has been designated "PlPV."

Original languageEnglish (US)
Pages (from-to)61-70
Number of pages10
JournalJournal of Zoo and Wildlife Medicine
Volume27
Issue number1
StatePublished - Mar 1996

Fingerprint

Lions
Papillomaviridae
Panthera leo
hyperplasia
lesion
Hyperplasia
mouth
Epitopes
Cats
lesions (animal)
DNA
Skin
Inclusion Bodies
Felidae
DNA Restriction Enzymes
Viral Proteins
Southern Blotting
antigen
electron microscopy
Cell Nucleus

Keywords

  • Asian lion
  • Felidae
  • Panthera leo persica
  • Papillomavirus
  • PlPV

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Environmental Science(all)
  • veterinary(all)

Cite this

Sundberg, J. P., Montali, R. J., Bush, M., Phillips, L. G., O'Brien, S. J., Jenson, A. B., ... Van Ranst, M. (1996). Papillomavirus-associated focal oral hyperplasia in wild and captive asian lions (Panthera leo persica). Journal of Zoo and Wildlife Medicine, 27(1), 61-70.

Papillomavirus-associated focal oral hyperplasia in wild and captive asian lions (Panthera leo persica). / Sundberg, John P.; Montali, Richard J.; Bush, Mitchell; Phillips, Lyndsay G.; O'Brien, Stephen J.; Jenson, A. Bennett; Burk, Robert D.; Van Ranst, Marc.

In: Journal of Zoo and Wildlife Medicine, Vol. 27, No. 1, 03.1996, p. 61-70.

Research output: Contribution to journalArticle

Sundberg, JP, Montali, RJ, Bush, M, Phillips, LG, O'Brien, SJ, Jenson, AB, Burk, RD & Van Ranst, M 1996, 'Papillomavirus-associated focal oral hyperplasia in wild and captive asian lions (Panthera leo persica)', Journal of Zoo and Wildlife Medicine, vol. 27, no. 1, pp. 61-70.
Sundberg JP, Montali RJ, Bush M, Phillips LG, O'Brien SJ, Jenson AB et al. Papillomavirus-associated focal oral hyperplasia in wild and captive asian lions (Panthera leo persica). Journal of Zoo and Wildlife Medicine. 1996 Mar;27(1):61-70.
Sundberg, John P. ; Montali, Richard J. ; Bush, Mitchell ; Phillips, Lyndsay G. ; O'Brien, Stephen J. ; Jenson, A. Bennett ; Burk, Robert D. ; Van Ranst, Marc. / Papillomavirus-associated focal oral hyperplasia in wild and captive asian lions (Panthera leo persica). In: Journal of Zoo and Wildlife Medicine. 1996 ; Vol. 27, No. 1. pp. 61-70.
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