Palmitoylation of a G protein αi subunit requires membrane localization not myristoylation

Michael Y. Degtyarev, Allen M. Spiegel, Teresa L Z Jones

Research output: Contribution to journalArticle

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Abstract

Palmitoylation is a dynamic, post-translational modification of the amino terminus of heterotrimeric G protein α subunits. Since myristoylation, βγ interactions, and membrane attachment also involve the amino terminus of the G protein αil subunit, we studied the relationships between palmitoylation and these events. Using COS cell transfection, the turnover of palmitate was slower on αil subunits co-expressed with β and γ subunits than on the αil subunit expressed alone. Mutation of cysteine 3 of αil prevented [3H]palmitate but not [3H]myristate incorporation and decreased the membrane localization of this protein. This nonpalmitoylated mutant could form a heterotrimer with co-expressed βγ subunits which restored its membrane localization. A nonmyristoylated αil mutant (glycine 2 to alanine) could incorporate [3H]palmitate when co-expressed with βγ subunits and localized to the membrane. The [3H]palmitate turnover of this nonmyristoylated mutant was more rapid than seen with the wild-type αil subunit. While myristoylation is not required for palmitoylation, both myristoylation and βγ association can slow the turnover of palmitate on αil. These results suggest that palmitoylation maintains the membrane attachment of the free α subunit and changes in βγ association could modulate palmitoylation during signaling.

Original languageEnglish (US)
Pages (from-to)30898-30903
Number of pages6
JournalJournal of Biological Chemistry
Volume269
Issue number49
StatePublished - Dec 9 1994
Externally publishedYes

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Lipoylation
Palmitates
Protein Subunits
GTP-Binding Proteins
Membranes
Heterotrimeric GTP-Binding Proteins
COS Cells
Myristic Acid
Post Translational Protein Processing
Alanine
Glycine
Transfection
Cysteine
Membrane Proteins
Mutation
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Palmitoylation of a G protein αi subunit requires membrane localization not myristoylation. / Degtyarev, Michael Y.; Spiegel, Allen M.; Jones, Teresa L Z.

In: Journal of Biological Chemistry, Vol. 269, No. 49, 09.12.1994, p. 30898-30903.

Research output: Contribution to journalArticle

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