Palm is expressed in both developing and adult mouse lens and retina

Meryl Castellini, Louise V. Wolf, Bharesh K. Chauhan, Deni S. Galileo, Manfred W. Kilimann, Ales Cvekl, Melinda K. Duncan

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: Paralemmin (Palm) is a prenyl-palmitoyl anchored membrane protein that can drive membrane and process formation in neurons. Earlier studies have shown brain preferred Palm expression, although this protein is a major water insoluble protein in chicken lens fiber cells and the Palm gene may be regulated by Pax6. Methods: The expression profile of Palm protein in the embryonic, newborn and adult mouse eye as well as dissociated retinal neurons was determined by confocal immunofluorescence. The relative mRNA levels of Palm, Palmdelphin (PalmD) and paralemmin2 (Palm2) in the lens and retina were determined by real time rt-PCR. Results: In the lens, Palm is already expressed at 9.5 dpc in the lens placode, and this expression is maintained in the lens vesicle throughout the formation of the adult lens. Palm is largely absent from the optic vesicle but is detectable at 10.5 dpc in the optic cup. In the developing retina, Palm expression transiently upregulates during the formation of optic nerve as well as in the formation of both the inner and outer plexiform layers. In short term dissociated chick retinal cultures, Palm protein is easily detectable, but the levels appear to reduce sharply as the cultures age. Palm mRNA was found at much higher levels relative to Palm2 or PalmD in both the retina and lens. Conclusion: Palm is the major paralemmin family member expressed in the retina and lens and its expression in the retina transiently upregulates during active neurite outgrowth. The expression pattern of Palm in the eye is consistent with it being a Pax6 responsive gene. Since Palm is known to be able to drive membrane formation in brain neurons, it is possible that this molecule is crucial for the increase in membrane formation during lens fiber cell differentiation.

Original languageEnglish (US)
Article number14
JournalBMC Ophthalmology
Volume5
DOIs
StatePublished - Jun 21 2005

Fingerprint

Lenses
Retina
Membranes
Proteins
Up-Regulation
Retinal Neurons
Neurons
Messenger RNA
Brain
Optic Nerve
Genes
Fluorescent Antibody Technique
Real-Time Polymerase Chain Reaction
Cell Differentiation
Chickens
Membrane Proteins
Newborn Infant
Water

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Castellini, M., Wolf, L. V., Chauhan, B. K., Galileo, D. S., Kilimann, M. W., Cvekl, A., & Duncan, M. K. (2005). Palm is expressed in both developing and adult mouse lens and retina. BMC Ophthalmology, 5, [14]. https://doi.org/10.1186/1471-2415-5-14

Palm is expressed in both developing and adult mouse lens and retina. / Castellini, Meryl; Wolf, Louise V.; Chauhan, Bharesh K.; Galileo, Deni S.; Kilimann, Manfred W.; Cvekl, Ales; Duncan, Melinda K.

In: BMC Ophthalmology, Vol. 5, 14, 21.06.2005.

Research output: Contribution to journalArticle

Castellini, M, Wolf, LV, Chauhan, BK, Galileo, DS, Kilimann, MW, Cvekl, A & Duncan, MK 2005, 'Palm is expressed in both developing and adult mouse lens and retina', BMC Ophthalmology, vol. 5, 14. https://doi.org/10.1186/1471-2415-5-14
Castellini M, Wolf LV, Chauhan BK, Galileo DS, Kilimann MW, Cvekl A et al. Palm is expressed in both developing and adult mouse lens and retina. BMC Ophthalmology. 2005 Jun 21;5. 14. https://doi.org/10.1186/1471-2415-5-14
Castellini, Meryl ; Wolf, Louise V. ; Chauhan, Bharesh K. ; Galileo, Deni S. ; Kilimann, Manfred W. ; Cvekl, Ales ; Duncan, Melinda K. / Palm is expressed in both developing and adult mouse lens and retina. In: BMC Ophthalmology. 2005 ; Vol. 5.
@article{013722e747f845ebaf8fb6fa6935b047,
title = "Palm is expressed in both developing and adult mouse lens and retina",
abstract = "Background: Paralemmin (Palm) is a prenyl-palmitoyl anchored membrane protein that can drive membrane and process formation in neurons. Earlier studies have shown brain preferred Palm expression, although this protein is a major water insoluble protein in chicken lens fiber cells and the Palm gene may be regulated by Pax6. Methods: The expression profile of Palm protein in the embryonic, newborn and adult mouse eye as well as dissociated retinal neurons was determined by confocal immunofluorescence. The relative mRNA levels of Palm, Palmdelphin (PalmD) and paralemmin2 (Palm2) in the lens and retina were determined by real time rt-PCR. Results: In the lens, Palm is already expressed at 9.5 dpc in the lens placode, and this expression is maintained in the lens vesicle throughout the formation of the adult lens. Palm is largely absent from the optic vesicle but is detectable at 10.5 dpc in the optic cup. In the developing retina, Palm expression transiently upregulates during the formation of optic nerve as well as in the formation of both the inner and outer plexiform layers. In short term dissociated chick retinal cultures, Palm protein is easily detectable, but the levels appear to reduce sharply as the cultures age. Palm mRNA was found at much higher levels relative to Palm2 or PalmD in both the retina and lens. Conclusion: Palm is the major paralemmin family member expressed in the retina and lens and its expression in the retina transiently upregulates during active neurite outgrowth. The expression pattern of Palm in the eye is consistent with it being a Pax6 responsive gene. Since Palm is known to be able to drive membrane formation in brain neurons, it is possible that this molecule is crucial for the increase in membrane formation during lens fiber cell differentiation.",
author = "Meryl Castellini and Wolf, {Louise V.} and Chauhan, {Bharesh K.} and Galileo, {Deni S.} and Kilimann, {Manfred W.} and Ales Cvekl and Duncan, {Melinda K.}",
year = "2005",
month = "6",
day = "21",
doi = "10.1186/1471-2415-5-14",
language = "English (US)",
volume = "5",
journal = "BMC Ophthalmology",
issn = "1471-2415",
publisher = "BioMed Central",

}

TY - JOUR

T1 - Palm is expressed in both developing and adult mouse lens and retina

AU - Castellini, Meryl

AU - Wolf, Louise V.

AU - Chauhan, Bharesh K.

AU - Galileo, Deni S.

AU - Kilimann, Manfred W.

AU - Cvekl, Ales

AU - Duncan, Melinda K.

PY - 2005/6/21

Y1 - 2005/6/21

N2 - Background: Paralemmin (Palm) is a prenyl-palmitoyl anchored membrane protein that can drive membrane and process formation in neurons. Earlier studies have shown brain preferred Palm expression, although this protein is a major water insoluble protein in chicken lens fiber cells and the Palm gene may be regulated by Pax6. Methods: The expression profile of Palm protein in the embryonic, newborn and adult mouse eye as well as dissociated retinal neurons was determined by confocal immunofluorescence. The relative mRNA levels of Palm, Palmdelphin (PalmD) and paralemmin2 (Palm2) in the lens and retina were determined by real time rt-PCR. Results: In the lens, Palm is already expressed at 9.5 dpc in the lens placode, and this expression is maintained in the lens vesicle throughout the formation of the adult lens. Palm is largely absent from the optic vesicle but is detectable at 10.5 dpc in the optic cup. In the developing retina, Palm expression transiently upregulates during the formation of optic nerve as well as in the formation of both the inner and outer plexiform layers. In short term dissociated chick retinal cultures, Palm protein is easily detectable, but the levels appear to reduce sharply as the cultures age. Palm mRNA was found at much higher levels relative to Palm2 or PalmD in both the retina and lens. Conclusion: Palm is the major paralemmin family member expressed in the retina and lens and its expression in the retina transiently upregulates during active neurite outgrowth. The expression pattern of Palm in the eye is consistent with it being a Pax6 responsive gene. Since Palm is known to be able to drive membrane formation in brain neurons, it is possible that this molecule is crucial for the increase in membrane formation during lens fiber cell differentiation.

AB - Background: Paralemmin (Palm) is a prenyl-palmitoyl anchored membrane protein that can drive membrane and process formation in neurons. Earlier studies have shown brain preferred Palm expression, although this protein is a major water insoluble protein in chicken lens fiber cells and the Palm gene may be regulated by Pax6. Methods: The expression profile of Palm protein in the embryonic, newborn and adult mouse eye as well as dissociated retinal neurons was determined by confocal immunofluorescence. The relative mRNA levels of Palm, Palmdelphin (PalmD) and paralemmin2 (Palm2) in the lens and retina were determined by real time rt-PCR. Results: In the lens, Palm is already expressed at 9.5 dpc in the lens placode, and this expression is maintained in the lens vesicle throughout the formation of the adult lens. Palm is largely absent from the optic vesicle but is detectable at 10.5 dpc in the optic cup. In the developing retina, Palm expression transiently upregulates during the formation of optic nerve as well as in the formation of both the inner and outer plexiform layers. In short term dissociated chick retinal cultures, Palm protein is easily detectable, but the levels appear to reduce sharply as the cultures age. Palm mRNA was found at much higher levels relative to Palm2 or PalmD in both the retina and lens. Conclusion: Palm is the major paralemmin family member expressed in the retina and lens and its expression in the retina transiently upregulates during active neurite outgrowth. The expression pattern of Palm in the eye is consistent with it being a Pax6 responsive gene. Since Palm is known to be able to drive membrane formation in brain neurons, it is possible that this molecule is crucial for the increase in membrane formation during lens fiber cell differentiation.

UR - http://www.scopus.com/inward/record.url?scp=23944524385&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=23944524385&partnerID=8YFLogxK

U2 - 10.1186/1471-2415-5-14

DO - 10.1186/1471-2415-5-14

M3 - Article

C2 - 15969763

AN - SCOPUS:23944524385

VL - 5

JO - BMC Ophthalmology

JF - BMC Ophthalmology

SN - 1471-2415

M1 - 14

ER -