P2Y1 receptor activation enhances the rate of rat pinealocyte-induced extracellular acidification via a calcium-dependent mechanism

Zulma Silva Ferreira, Célia Regina S. Garcia, David C. Spray, Regina P. Markus

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Pineal gland G-protein coupled P2Y1 receptors potentiate noradrenaline-induced N′-acetylserotonin production, a long term response which occurs after 5 h incubation. In the current study we show that a short-term effect of stimulation of P2Y1 receptors is the increase in extracellular acidification rate (ECAR), which is mediated by an increase in intracellular calcium concentration ([Ca2+]i). The pD2 values for ATP (3.06 ± 0.12)-induced ECAR increase was significantly smaller (p < 0.01) than that for ADP (3.64 ± 0.18), 2MeSATP (3.56 ± 0.02) and 2MeSADP (3.65 ± 0.13). The selective P2Y1 receptorantagonists A3′P-5′P and A3′P-5′PS inhibited the increase in ECAR-induced by ADP. Clamping [Ca2+]i with BAPTA (30 and 50 μmol/l) led to inhibition of ADP-induced increase in ECAR. Agonist and antagonist data indicate P2Y1 activation leads to a [Ca2+]i-dependent acidification of the extracellular medium.

Original languageEnglish (US)
Pages (from-to)33-37
Number of pages5
JournalPharmacology
Volume69
Issue number1
DOIs
Publication statusPublished - Aug 11 2003

    Fingerprint

Keywords

  • Intracellular Ca
  • Microphysiometry
  • P2Y receptor
  • Pinealocytes

ASJC Scopus subject areas

  • Pharmacology

Cite this