P-glycoprotein transports corticosterone and is photoaffinity-labeled by the steroid

D. C. Wolf, Susan Band Horwitz

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Multi-drug-resistant cells overproduce a 130-180-kDa integral membrane phosphoglycoprotein known as P-glycoprotein which acts as an energy-dependent drug efflux pump. While P-glycoprotein has been shown to transport hydrophobic antitumor drugs out of multi-drug-resistant cells in tissue culture, its endogenous substrates remain unknown. This report shows that 3H-corticosterone can specifically photoaffinity label P-glycoprotein. Furthermore, corticosterone is effluxed from multidrug-resistant cells by P-glycoprotein. These data suggest that corticosterone may be an endogenous substrate for P-glycoprotein.

Original languageEnglish (US)
Pages (from-to)141-146
Number of pages6
JournalInternational Journal of Cancer
Volume52
Issue number1
StatePublished - 1992

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P-Glycoprotein
Corticosterone
Steroids
Photoaffinity Labels
Pharmaceutical Preparations
Antineoplastic Agents
Membranes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

P-glycoprotein transports corticosterone and is photoaffinity-labeled by the steroid. / Wolf, D. C.; Band Horwitz, Susan.

In: International Journal of Cancer, Vol. 52, No. 1, 1992, p. 141-146.

Research output: Contribution to journalArticle

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