TY - JOUR
T1 - Ozonation of dna forms adducts
T2 - A 32p-dna labeling and thin-layer chromatography technique to measure dna environmental biomarkers
AU - Cajigas, Antonio
AU - Gayer, Mitchell
AU - Beam, Carl
AU - Steinberg, J. J.
N1 - Funding Information:
This research was supported in part by the American Foundation for Aging Research and a grant-in-aide from the Lauren and I. Ezra Merkin Fund of the Tehilla Foundation.
PY - 1994
Y1 - 1994
N2 - Little direct documented evidence of ozone's genotoxicity exists. Deoxyribonucleic acid (DNA) adducts are produced by environmental toxic agents, including ozone. We have described a modified thin-layer chromatography (TLC) technique that can assess adduct formation as a biomarker of ozone injury. This requires 32P-labeling DNA, digestion of deoxynucleotides (dNMPs), and separation in two-dimensional PEI-cellulose TLC. We have applied this technique to control DNAs, to control DNA in solution exposed to acute ambient ozone, and to control DNA exposed to acute bubbled-through ozone (2 ppm for 24 h). We detected stable DNA adducts, including hydroxymethyluracil (HMU), thymine glycol (TC), 8-hydroxyguanine (8–OHG), and demonstrated, as yet, unidentified adducts that may serve as a “fingerprint” pattern of DNA adduction. This technique quantifies low-molecularmass DNA adducts, both in vivo and in vitro, with potential applications to environmental toxicology.
AB - Little direct documented evidence of ozone's genotoxicity exists. Deoxyribonucleic acid (DNA) adducts are produced by environmental toxic agents, including ozone. We have described a modified thin-layer chromatography (TLC) technique that can assess adduct formation as a biomarker of ozone injury. This requires 32P-labeling DNA, digestion of deoxynucleotides (dNMPs), and separation in two-dimensional PEI-cellulose TLC. We have applied this technique to control DNAs, to control DNA in solution exposed to acute ambient ozone, and to control DNA exposed to acute bubbled-through ozone (2 ppm for 24 h). We detected stable DNA adducts, including hydroxymethyluracil (HMU), thymine glycol (TC), 8-hydroxyguanine (8–OHG), and demonstrated, as yet, unidentified adducts that may serve as a “fingerprint” pattern of DNA adduction. This technique quantifies low-molecularmass DNA adducts, both in vivo and in vitro, with potential applications to environmental toxicology.
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U2 - 10.1080/00039896.1994.9934411
DO - 10.1080/00039896.1994.9934411
M3 - Article
C2 - 8117143
AN - SCOPUS:0027955251
SN - 0003-9896
VL - 49
SP - 25
EP - 36
JO - Archives of Environmental Health
JF - Archives of Environmental Health
IS - 1
ER -
