Overproduction of urokinase-type plasminogen activator is regulated by phospholipase D- and protein kinase C-dependent pathways in murine mammary adenocarcinoma cells

Julio A. Aguirre Ghiso, Daniel F. Alonso, Eduardo F. Farías, Elisa Bal de Kier Joffé

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Urokinase-type plasminogen activator (uPA) initiates a proteolytic cascade with which invasive cells eliminate barriers to movement. The signaling pathways regulating uPA production in tumor cells remain unclear. We first studied the effects of n-butanol, a phospholipase D (PLD) and protein kinase C (PKC) inhibitor, on the production of uPA in murine mammary adenocarcinoma cells. Tumor cell monolayers treated during 24 h with 0.3% v/v n-butanol, secreted 45-50% less uPA to the culture medium than control monolayers (P < 0.001) as determined by radial caseinolysis, zymography and western blot. This inhibition occurred also with 5-h treatments and remained up to 5 h after the removal of the alcohol. Treatment with the phorbol ester PMA or with EGF, strongly increased uPA production(P < 0.001). Interestingly, a mild inhibition of uPA production was observed when PMA stimulation was assayed in cotreatments with n-butanol. In contrast EGF was unable to reverse the inhibition induced by 12-butanol. H7 significantly inhibited uPA activity (P < 0.001) secreted to the culture media. Furthermore, phosphatidic acid significantly stimulated uPA production meanwhile propranolol, which blocks phosphatidic acid availability, reduced it, suggesting a main regulatory role for this intermediary metabolite. These results suggest for the first time that uPA production is regulated by PLD and PKC signal transduction pathways in murine mammary adenocarcinoma cells.

Original languageEnglish (US)
Pages (from-to)171-184
Number of pages14
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1356
Issue number2
DOIs
StatePublished - Apr 24 1997
Externally publishedYes

Keywords

  • Invasion
  • Metastasis
  • Phosphatidic acid phosphohydrolase
  • Phosphtidic acid
  • PKC
  • PLD
  • Propranolol
  • Protease
  • Signal transduction
  • Tumor cell

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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