Overlapping functions of E- and P-selectin in neutrophil recruitment during acute inflammation

Jonathon W. Homeister, Mengkun Zhang, Paul S. Frenette, Richard O. Hynes, Denisa D. Wagner, John B. Lowe, Rory M. Marks

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Selectin adhesion molecules mediate leukocyte rolling on activated endothelium, a prerequisite to leukocyte accumulation at sites of inflammation. The precise role of each selectin (E-, P-, and L-) in this process is unclear and may vary depending on the particular inflammatory stimulus, vascular bed, leukocyte subset, and species; most data suggest discrete functional roles for each selectin. To define the relative roles of E- and P-selectin in mediating neutrophil accumulation in acute dermal inflammation, mice genetically deficient in E-selectin, P-selectin, or both E- and P-selectin were injected intradermally with zymosan. Luminal endothelial expression of E- and P-selectin in response to zymosan was documented in wild-type mice by intravenous administration of fluorochrome- labeled anti-E- and anti-Pselectin antibodies. In mice deficient in E- or P- selectin, neutrophil accumulation was unchanged or only subtly reduced relative to wild-type control mice. In mice deficient in both E- and P- selectin, neutrophil accumulation was significantly reduced (87% at 4 hours and 79% at 8 hours). These data demonstrate that, in this model of acute inflammation, there is considerable overlap in the functions of E- and P- selectin; loss of both selectins was required to impair neutrophil accumulation.

Original languageEnglish (US)
Pages (from-to)2345-2352
Number of pages8
JournalBlood
Volume92
Issue number7
StatePublished - Oct 1 1998
Externally publishedYes

Fingerprint

P-Selectin
E-Selectin
Neutrophil Infiltration
Inflammation
Selectins
Neutrophils
Zymosan
Leukocytes
Leukocyte Rolling
L-Selectin
Cell Adhesion Molecules
Fluorescent Dyes
Intravenous Administration
Endothelium
Blood Vessels
Anti-Idiotypic Antibodies
Skin

ASJC Scopus subject areas

  • Hematology

Cite this

Homeister, J. W., Zhang, M., Frenette, P. S., Hynes, R. O., Wagner, D. D., Lowe, J. B., & Marks, R. M. (1998). Overlapping functions of E- and P-selectin in neutrophil recruitment during acute inflammation. Blood, 92(7), 2345-2352.

Overlapping functions of E- and P-selectin in neutrophil recruitment during acute inflammation. / Homeister, Jonathon W.; Zhang, Mengkun; Frenette, Paul S.; Hynes, Richard O.; Wagner, Denisa D.; Lowe, John B.; Marks, Rory M.

In: Blood, Vol. 92, No. 7, 01.10.1998, p. 2345-2352.

Research output: Contribution to journalArticle

Homeister, JW, Zhang, M, Frenette, PS, Hynes, RO, Wagner, DD, Lowe, JB & Marks, RM 1998, 'Overlapping functions of E- and P-selectin in neutrophil recruitment during acute inflammation', Blood, vol. 92, no. 7, pp. 2345-2352.
Homeister JW, Zhang M, Frenette PS, Hynes RO, Wagner DD, Lowe JB et al. Overlapping functions of E- and P-selectin in neutrophil recruitment during acute inflammation. Blood. 1998 Oct 1;92(7):2345-2352.
Homeister, Jonathon W. ; Zhang, Mengkun ; Frenette, Paul S. ; Hynes, Richard O. ; Wagner, Denisa D. ; Lowe, John B. ; Marks, Rory M. / Overlapping functions of E- and P-selectin in neutrophil recruitment during acute inflammation. In: Blood. 1998 ; Vol. 92, No. 7. pp. 2345-2352.
@article{1635e9fb51204d479b2cf91efe08a4bc,
title = "Overlapping functions of E- and P-selectin in neutrophil recruitment during acute inflammation",
abstract = "Selectin adhesion molecules mediate leukocyte rolling on activated endothelium, a prerequisite to leukocyte accumulation at sites of inflammation. The precise role of each selectin (E-, P-, and L-) in this process is unclear and may vary depending on the particular inflammatory stimulus, vascular bed, leukocyte subset, and species; most data suggest discrete functional roles for each selectin. To define the relative roles of E- and P-selectin in mediating neutrophil accumulation in acute dermal inflammation, mice genetically deficient in E-selectin, P-selectin, or both E- and P-selectin were injected intradermally with zymosan. Luminal endothelial expression of E- and P-selectin in response to zymosan was documented in wild-type mice by intravenous administration of fluorochrome- labeled anti-E- and anti-Pselectin antibodies. In mice deficient in E- or P- selectin, neutrophil accumulation was unchanged or only subtly reduced relative to wild-type control mice. In mice deficient in both E- and P- selectin, neutrophil accumulation was significantly reduced (87{\%} at 4 hours and 79{\%} at 8 hours). These data demonstrate that, in this model of acute inflammation, there is considerable overlap in the functions of E- and P- selectin; loss of both selectins was required to impair neutrophil accumulation.",
author = "Homeister, {Jonathon W.} and Mengkun Zhang and Frenette, {Paul S.} and Hynes, {Richard O.} and Wagner, {Denisa D.} and Lowe, {John B.} and Marks, {Rory M.}",
year = "1998",
month = "10",
day = "1",
language = "English (US)",
volume = "92",
pages = "2345--2352",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "7",

}

TY - JOUR

T1 - Overlapping functions of E- and P-selectin in neutrophil recruitment during acute inflammation

AU - Homeister, Jonathon W.

AU - Zhang, Mengkun

AU - Frenette, Paul S.

AU - Hynes, Richard O.

AU - Wagner, Denisa D.

AU - Lowe, John B.

AU - Marks, Rory M.

PY - 1998/10/1

Y1 - 1998/10/1

N2 - Selectin adhesion molecules mediate leukocyte rolling on activated endothelium, a prerequisite to leukocyte accumulation at sites of inflammation. The precise role of each selectin (E-, P-, and L-) in this process is unclear and may vary depending on the particular inflammatory stimulus, vascular bed, leukocyte subset, and species; most data suggest discrete functional roles for each selectin. To define the relative roles of E- and P-selectin in mediating neutrophil accumulation in acute dermal inflammation, mice genetically deficient in E-selectin, P-selectin, or both E- and P-selectin were injected intradermally with zymosan. Luminal endothelial expression of E- and P-selectin in response to zymosan was documented in wild-type mice by intravenous administration of fluorochrome- labeled anti-E- and anti-Pselectin antibodies. In mice deficient in E- or P- selectin, neutrophil accumulation was unchanged or only subtly reduced relative to wild-type control mice. In mice deficient in both E- and P- selectin, neutrophil accumulation was significantly reduced (87% at 4 hours and 79% at 8 hours). These data demonstrate that, in this model of acute inflammation, there is considerable overlap in the functions of E- and P- selectin; loss of both selectins was required to impair neutrophil accumulation.

AB - Selectin adhesion molecules mediate leukocyte rolling on activated endothelium, a prerequisite to leukocyte accumulation at sites of inflammation. The precise role of each selectin (E-, P-, and L-) in this process is unclear and may vary depending on the particular inflammatory stimulus, vascular bed, leukocyte subset, and species; most data suggest discrete functional roles for each selectin. To define the relative roles of E- and P-selectin in mediating neutrophil accumulation in acute dermal inflammation, mice genetically deficient in E-selectin, P-selectin, or both E- and P-selectin were injected intradermally with zymosan. Luminal endothelial expression of E- and P-selectin in response to zymosan was documented in wild-type mice by intravenous administration of fluorochrome- labeled anti-E- and anti-Pselectin antibodies. In mice deficient in E- or P- selectin, neutrophil accumulation was unchanged or only subtly reduced relative to wild-type control mice. In mice deficient in both E- and P- selectin, neutrophil accumulation was significantly reduced (87% at 4 hours and 79% at 8 hours). These data demonstrate that, in this model of acute inflammation, there is considerable overlap in the functions of E- and P- selectin; loss of both selectins was required to impair neutrophil accumulation.

UR - http://www.scopus.com/inward/record.url?scp=0032189827&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032189827&partnerID=8YFLogxK

M3 - Article

VL - 92

SP - 2345

EP - 2352

JO - Blood

JF - Blood

SN - 0006-4971

IS - 7

ER -