Overexpression of IL-1 receptor accessory protein in stem and progenitor cells and outcome correlation in AML and MDS

Laura Barreyro, Britta Will, Boris A. Bartholdy, Li Zhou, Tihomira I. Todorova, Robert F. Stanley, Susana Ben-Neriah, Cristina Montagna, Samir Parekh, Andrea Pellagatti, Jacqueline Boultwood, Elisabeth M. Paietta, Rhett P. Ketterling, Larry Cripe, Hugo F. Fernandez, Peter L. Greenberg, Martin S. Tallman, Christian Steidl, Constantine S. Mitsiades, Amit K. VermaUlrich G. Steidl

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Cellular and interpatient heterogeneity and the involvement of different stem and progenitor compartments in leukemogenesis are challenges for the identification of common pathways contributing to the initiation and maintenance of acute myeloid leukemia (AML). Here we used a strategy of parallel transcriptional analysis of phenotypic long-term hematopoietic stem cells (HSCs), short-term HSCs, and granulocyte-monocyte progenitors from individuals with high-risk (-7/7q-) AML and compared them with the corresponding cell populations from healthy controls. This analysis revealed dysregulated expression of 11 genes, including IL-1 receptor accessory protein (IL1RAP), in all leukemic stem and progenitor cell compartments. IL1RAP protein was found to be overexpressed on the surface of HSCs of AML patients, and marked cells with the -7/7q- anomaly. IL1RAP was also overexpressed on HSCs of patients with normal karyotype AML and high-risk myelodysplastic syndrome, suggesting a pervasive role in different disease subtypes. High IL1RAP expression was independently associated with poor overall survival in 3 independent cohorts of AML patients (P = 2.2 × 10-7). Knockdown of IL1RAP decreased clonogenicity and increased cell death ofAML cells. Our study identified genes dysregulated in stem and progenitor cells in -7/7q- AML, and suggests that IL1RAP may be a promising therapeutic and prognostic target in AML and high-risk myelodysplastic syndrome.

Original languageEnglish (US)
Pages (from-to)1290-1298
Number of pages9
JournalBlood
Volume120
Issue number6
DOIs
StatePublished - Aug 9 2012

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Interleukin-1 Receptors
Accessories
Acute Myeloid Leukemia
Stem Cells
Hematopoietic Stem Cells
Stem cells
Proteins
Myelodysplastic Syndromes
Genes
Granulocyte Precursor Cells
Population Control
Cell death
Karyotype
Monocytes
Cell Death
Cells
Maintenance
Gene Expression
Survival

ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology

Cite this

Overexpression of IL-1 receptor accessory protein in stem and progenitor cells and outcome correlation in AML and MDS. / Barreyro, Laura; Will, Britta; Bartholdy, Boris A.; Zhou, Li; Todorova, Tihomira I.; Stanley, Robert F.; Ben-Neriah, Susana; Montagna, Cristina; Parekh, Samir; Pellagatti, Andrea; Boultwood, Jacqueline; Paietta, Elisabeth M.; Ketterling, Rhett P.; Cripe, Larry; Fernandez, Hugo F.; Greenberg, Peter L.; Tallman, Martin S.; Steidl, Christian; Mitsiades, Constantine S.; Verma, Amit K.; Steidl, Ulrich G.

In: Blood, Vol. 120, No. 6, 09.08.2012, p. 1290-1298.

Research output: Contribution to journalArticle

Barreyro, L, Will, B, Bartholdy, BA, Zhou, L, Todorova, TI, Stanley, RF, Ben-Neriah, S, Montagna, C, Parekh, S, Pellagatti, A, Boultwood, J, Paietta, EM, Ketterling, RP, Cripe, L, Fernandez, HF, Greenberg, PL, Tallman, MS, Steidl, C, Mitsiades, CS, Verma, AK & Steidl, UG 2012, 'Overexpression of IL-1 receptor accessory protein in stem and progenitor cells and outcome correlation in AML and MDS', Blood, vol. 120, no. 6, pp. 1290-1298. https://doi.org/10.1182/blood-2012-01-404699
Barreyro, Laura ; Will, Britta ; Bartholdy, Boris A. ; Zhou, Li ; Todorova, Tihomira I. ; Stanley, Robert F. ; Ben-Neriah, Susana ; Montagna, Cristina ; Parekh, Samir ; Pellagatti, Andrea ; Boultwood, Jacqueline ; Paietta, Elisabeth M. ; Ketterling, Rhett P. ; Cripe, Larry ; Fernandez, Hugo F. ; Greenberg, Peter L. ; Tallman, Martin S. ; Steidl, Christian ; Mitsiades, Constantine S. ; Verma, Amit K. ; Steidl, Ulrich G. / Overexpression of IL-1 receptor accessory protein in stem and progenitor cells and outcome correlation in AML and MDS. In: Blood. 2012 ; Vol. 120, No. 6. pp. 1290-1298.
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abstract = "Cellular and interpatient heterogeneity and the involvement of different stem and progenitor compartments in leukemogenesis are challenges for the identification of common pathways contributing to the initiation and maintenance of acute myeloid leukemia (AML). Here we used a strategy of parallel transcriptional analysis of phenotypic long-term hematopoietic stem cells (HSCs), short-term HSCs, and granulocyte-monocyte progenitors from individuals with high-risk (-7/7q-) AML and compared them with the corresponding cell populations from healthy controls. This analysis revealed dysregulated expression of 11 genes, including IL-1 receptor accessory protein (IL1RAP), in all leukemic stem and progenitor cell compartments. IL1RAP protein was found to be overexpressed on the surface of HSCs of AML patients, and marked cells with the -7/7q- anomaly. IL1RAP was also overexpressed on HSCs of patients with normal karyotype AML and high-risk myelodysplastic syndrome, suggesting a pervasive role in different disease subtypes. High IL1RAP expression was independently associated with poor overall survival in 3 independent cohorts of AML patients (P = 2.2 × 10-7). Knockdown of IL1RAP decreased clonogenicity and increased cell death ofAML cells. Our study identified genes dysregulated in stem and progenitor cells in -7/7q- AML, and suggests that IL1RAP may be a promising therapeutic and prognostic target in AML and high-risk myelodysplastic syndrome.",
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T1 - Overexpression of IL-1 receptor accessory protein in stem and progenitor cells and outcome correlation in AML and MDS

AU - Barreyro, Laura

AU - Will, Britta

AU - Bartholdy, Boris A.

AU - Zhou, Li

AU - Todorova, Tihomira I.

AU - Stanley, Robert F.

AU - Ben-Neriah, Susana

AU - Montagna, Cristina

AU - Parekh, Samir

AU - Pellagatti, Andrea

AU - Boultwood, Jacqueline

AU - Paietta, Elisabeth M.

AU - Ketterling, Rhett P.

AU - Cripe, Larry

AU - Fernandez, Hugo F.

AU - Greenberg, Peter L.

AU - Tallman, Martin S.

AU - Steidl, Christian

AU - Mitsiades, Constantine S.

AU - Verma, Amit K.

AU - Steidl, Ulrich G.

PY - 2012/8/9

Y1 - 2012/8/9

N2 - Cellular and interpatient heterogeneity and the involvement of different stem and progenitor compartments in leukemogenesis are challenges for the identification of common pathways contributing to the initiation and maintenance of acute myeloid leukemia (AML). Here we used a strategy of parallel transcriptional analysis of phenotypic long-term hematopoietic stem cells (HSCs), short-term HSCs, and granulocyte-monocyte progenitors from individuals with high-risk (-7/7q-) AML and compared them with the corresponding cell populations from healthy controls. This analysis revealed dysregulated expression of 11 genes, including IL-1 receptor accessory protein (IL1RAP), in all leukemic stem and progenitor cell compartments. IL1RAP protein was found to be overexpressed on the surface of HSCs of AML patients, and marked cells with the -7/7q- anomaly. IL1RAP was also overexpressed on HSCs of patients with normal karyotype AML and high-risk myelodysplastic syndrome, suggesting a pervasive role in different disease subtypes. High IL1RAP expression was independently associated with poor overall survival in 3 independent cohorts of AML patients (P = 2.2 × 10-7). Knockdown of IL1RAP decreased clonogenicity and increased cell death ofAML cells. Our study identified genes dysregulated in stem and progenitor cells in -7/7q- AML, and suggests that IL1RAP may be a promising therapeutic and prognostic target in AML and high-risk myelodysplastic syndrome.

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