Overexpression of E2F-1 in lung and liver metastases of human colon cancer is associated with gene amplification

Marian Iwamoto, Debabrata Banerjee, Lata G. Menon, Anna Jurkiewicz, Pulivarthi H. Rao, Nancy E. Kemeny, Yuman Fong, Suresh C. Jhanwar, Richard Gorlick, Joseph R. Bertino

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

We have shown previously that metastatic tumors of human colorectal cancer in lung as compared to liver have high levels of thymidylate synthase (TS) mRNA expression that correlated with high levels of E2F-1 mRNA expression. We now report that Comparative Genomic Hybridization (CGH) and DNA PCR analyses of lung and liver metastases of human colon cancer show frequent gains in the region of chromosome 20q and have an increase in gene copy number of E2F-1. In as much as TS is transcriptionally regulated by E2F-1, these results provide an explanation for the high levels of TS mRNA noted in some tumor samples.

Original languageEnglish (US)
Pages (from-to)395-399
Number of pages5
JournalCancer Biology and Therapy
Volume3
Issue number4
StatePublished - Apr 2004
Externally publishedYes

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Thymidylate Synthase
Gene Amplification
Colonic Neoplasms
Neoplasm Metastasis
Lung
Messenger RNA
Liver
Comparative Genomic Hybridization
Gene Dosage
Colorectal Neoplasms
Lung Neoplasms
Neoplasms
Chromosomes
Polymerase Chain Reaction
DNA

Keywords

  • 20q
  • Colorectal cancer
  • E2F-1 gene amplification
  • Metastases

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Iwamoto, M., Banerjee, D., Menon, L. G., Jurkiewicz, A., Rao, P. H., Kemeny, N. E., ... Bertino, J. R. (2004). Overexpression of E2F-1 in lung and liver metastases of human colon cancer is associated with gene amplification. Cancer Biology and Therapy, 3(4), 395-399.

Overexpression of E2F-1 in lung and liver metastases of human colon cancer is associated with gene amplification. / Iwamoto, Marian; Banerjee, Debabrata; Menon, Lata G.; Jurkiewicz, Anna; Rao, Pulivarthi H.; Kemeny, Nancy E.; Fong, Yuman; Jhanwar, Suresh C.; Gorlick, Richard; Bertino, Joseph R.

In: Cancer Biology and Therapy, Vol. 3, No. 4, 04.2004, p. 395-399.

Research output: Contribution to journalArticle

Iwamoto, M, Banerjee, D, Menon, LG, Jurkiewicz, A, Rao, PH, Kemeny, NE, Fong, Y, Jhanwar, SC, Gorlick, R & Bertino, JR 2004, 'Overexpression of E2F-1 in lung and liver metastases of human colon cancer is associated with gene amplification', Cancer Biology and Therapy, vol. 3, no. 4, pp. 395-399.
Iwamoto M, Banerjee D, Menon LG, Jurkiewicz A, Rao PH, Kemeny NE et al. Overexpression of E2F-1 in lung and liver metastases of human colon cancer is associated with gene amplification. Cancer Biology and Therapy. 2004 Apr;3(4):395-399.
Iwamoto, Marian ; Banerjee, Debabrata ; Menon, Lata G. ; Jurkiewicz, Anna ; Rao, Pulivarthi H. ; Kemeny, Nancy E. ; Fong, Yuman ; Jhanwar, Suresh C. ; Gorlick, Richard ; Bertino, Joseph R. / Overexpression of E2F-1 in lung and liver metastases of human colon cancer is associated with gene amplification. In: Cancer Biology and Therapy. 2004 ; Vol. 3, No. 4. pp. 395-399.
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