Overexpression of E2F-1 in lung and liver metastases of human colon cancer is associated with gene amplification

Marian Iwamoto, Debabrata Banerjee, Lata G. Menon, Anna Jurkiewicz, Pulivarthi H. Rao, Nancy E. Kemeny, Yuman Fong, Suresh C. Jhanwar, Richard Gorlick, Joseph R. Bertino

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

We have shown previously that metastatic tumors of human colorectal cancer in lung as compared to liver have high levels of thymidylate synthase (TS) mRNA expression that correlated with high levels of E2F-1 mRNA expression. We now report that Comparative Genomic Hybridization (CGH) and DNA PCR analyses of lung and liver metastases of human colon cancer show frequent gains in the region of chromosome 20q and have an increase in gene copy number of E2F-1. In as much as TS is transcriptionally regulated by E2F-1, these results provide an explanation for the high levels of TS mRNA noted in some tumor samples.

Original languageEnglish (US)
Pages (from-to)395-399
Number of pages5
JournalCancer Biology and Therapy
Volume3
Issue number4
DOIs
StatePublished - Apr 2004

Keywords

  • 20q
  • Colorectal cancer
  • E2F-1 gene amplification
  • Metastases

ASJC Scopus subject areas

  • Molecular Medicine
  • Oncology
  • Pharmacology
  • Cancer Research

Fingerprint Dive into the research topics of 'Overexpression of E2F-1 in lung and liver metastases of human colon cancer is associated with gene amplification'. Together they form a unique fingerprint.

  • Cite this

    Iwamoto, M., Banerjee, D., Menon, L. G., Jurkiewicz, A., Rao, P. H., Kemeny, N. E., Fong, Y., Jhanwar, S. C., Gorlick, R., & Bertino, J. R. (2004). Overexpression of E2F-1 in lung and liver metastases of human colon cancer is associated with gene amplification. Cancer Biology and Therapy, 3(4), 395-399. https://doi.org/10.4161/cbt.3.4.733