Overexpression of E2F-1 in lung and liver metastases of human colon cancer is associated with gene amplification

Marian Iwamoto; Debabrata Banerjee; Lata G. Menon; Anna Jurkiewicz; Pulivarthi H. Rao; Nancy E. Kemeny; Yuman Fong; Suresh C. Jhanwar; Richard Gorlick; Joseph R. Bertino

doi:10.4161/cbt.3.4.733

Overexpression of E2F-1 in lung and liver metastases of human colon cancer is associated with gene amplification

Marian Iwamoto, Debabrata Banerjee, Lata G. Menon, Anna Jurkiewicz, Pulivarthi H. Rao, Nancy E. Kemeny, Yuman Fong, Suresh C. Jhanwar, Richard Gorlick, Joseph R. Bertino

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

We have shown previously that metastatic tumors of human colorectal cancer in lung as compared to liver have high levels of thymidylate synthase (TS) mRNA expression that correlated with high levels of E2F-1 mRNA expression. We now report that Comparative Genomic Hybridization (CGH) and DNA PCR analyses of lung and liver metastases of human colon cancer show frequent gains in the region of chromosome 20q and have an increase in gene copy number of E2F-1. In as much as TS is transcriptionally regulated by E2F-1, these results provide an explanation for the high levels of TS mRNA noted in some tumor samples.

Original language	English (US)
Pages (from-to)	395-399
Number of pages	5
Journal	Cancer Biology and Therapy
Volume	3
Issue number	4
DOIs	https://doi.org/10.4161/cbt.3.4.733
State	Published - Apr 2004
Externally published	Yes

Keywords

20q
Colorectal cancer
E2F-1 gene amplification
Metastases

ASJC Scopus subject areas

Molecular Medicine
Oncology
Pharmacology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.4161/cbt.3.4.733

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title = "Overexpression of E2F-1 in lung and liver metastases of human colon cancer is associated with gene amplification",

abstract = "We have shown previously that metastatic tumors of human colorectal cancer in lung as compared to liver have high levels of thymidylate synthase (TS) mRNA expression that correlated with high levels of E2F-1 mRNA expression. We now report that Comparative Genomic Hybridization (CGH) and DNA PCR analyses of lung and liver metastases of human colon cancer show frequent gains in the region of chromosome 20q and have an increase in gene copy number of E2F-1. In as much as TS is transcriptionally regulated by E2F-1, these results provide an explanation for the high levels of TS mRNA noted in some tumor samples.",

keywords = "20q, Colorectal cancer, E2F-1 gene amplification, Metastases",

author = "Marian Iwamoto and Debabrata Banerjee and Menon, {Lata G.} and Anna Jurkiewicz and Rao, {Pulivarthi H.} and Kemeny, {Nancy E.} and Yuman Fong and Jhanwar, {Suresh C.} and Richard Gorlick and Bertino, {Joseph R.}",

note = "Funding Information: This work is supported by PHS award CA-61586",

year = "2004",

month = apr,

doi = "10.4161/cbt.3.4.733",

language = "English (US)",

volume = "3",

pages = "395--399",

journal = "Cancer Biology and Therapy",

issn = "1538-4047",

publisher = "Landes Bioscience",

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T1 - Overexpression of E2F-1 in lung and liver metastases of human colon cancer is associated with gene amplification

AU - Iwamoto, Marian

AU - Banerjee, Debabrata

AU - Menon, Lata G.

AU - Jurkiewicz, Anna

AU - Rao, Pulivarthi H.

AU - Kemeny, Nancy E.

AU - Fong, Yuman

AU - Jhanwar, Suresh C.

AU - Gorlick, Richard

AU - Bertino, Joseph R.

N1 - Funding Information: This work is supported by PHS award CA-61586

PY - 2004/4

Y1 - 2004/4

N2 - We have shown previously that metastatic tumors of human colorectal cancer in lung as compared to liver have high levels of thymidylate synthase (TS) mRNA expression that correlated with high levels of E2F-1 mRNA expression. We now report that Comparative Genomic Hybridization (CGH) and DNA PCR analyses of lung and liver metastases of human colon cancer show frequent gains in the region of chromosome 20q and have an increase in gene copy number of E2F-1. In as much as TS is transcriptionally regulated by E2F-1, these results provide an explanation for the high levels of TS mRNA noted in some tumor samples.

AB - We have shown previously that metastatic tumors of human colorectal cancer in lung as compared to liver have high levels of thymidylate synthase (TS) mRNA expression that correlated with high levels of E2F-1 mRNA expression. We now report that Comparative Genomic Hybridization (CGH) and DNA PCR analyses of lung and liver metastases of human colon cancer show frequent gains in the region of chromosome 20q and have an increase in gene copy number of E2F-1. In as much as TS is transcriptionally regulated by E2F-1, these results provide an explanation for the high levels of TS mRNA noted in some tumor samples.

KW - 20q

KW - Colorectal cancer

KW - E2F-1 gene amplification

KW - Metastases

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DO - 10.4161/cbt.3.4.733

M3 - Article

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AN - SCOPUS:16544374118

SN - 1538-4047

VL - 3

SP - 395

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JO - Cancer Biology and Therapy

JF - Cancer Biology and Therapy

IS - 4

ER -

Overexpression of E2F-1 in lung and liver metastases of human colon cancer is associated with gene amplification

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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