Ouabain stimulation of 45Ca++ accumulation in the rat pituitary

N. Fleischer, J. McMillin Wood

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Anterior pituitary tissue from rats was incubated at 37 C in Krebs' Ringer's bicarbonate in an atmosphere of 95% O2 and 5% CO2 with hourly changes of the incubation media. After a one hour preincubation, 45Ca++ was added to the media and its uptake by the pituitary tissue was determined. The digitalis glycoside, ouabain, in concentrations of 5 x 10-4 and 1 x 10-3 M significantly augmented pituitary 45Ca++. This effect was dose and time dependent. Ouabain did not alter the washout kinetics for 14C inulin or affect pituitary weights suggesting that the observed changes in 45Ca++ content were the result of increased 45Ca++ accumulation. The effect of ouabain on calcium flux may be related to the stimulation of ACTH and GH release by ouabain.

Original languageEnglish (US)
Pages (from-to)1555-1559
Number of pages5
JournalEndocrinology
Volume92
Issue number5
StatePublished - 1973
Externally publishedYes

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Ouabain
Digitalis Glycosides
Inulin
Bicarbonates
Atmosphere
Adrenocorticotropic Hormone
Calcium
Weights and Measures

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Fleischer, N., & McMillin Wood, J. (1973). Ouabain stimulation of 45Ca++ accumulation in the rat pituitary. Endocrinology, 92(5), 1555-1559.

Ouabain stimulation of 45Ca++ accumulation in the rat pituitary. / Fleischer, N.; McMillin Wood, J.

In: Endocrinology, Vol. 92, No. 5, 1973, p. 1555-1559.

Research output: Contribution to journalArticle

Fleischer, N & McMillin Wood, J 1973, 'Ouabain stimulation of 45Ca++ accumulation in the rat pituitary', Endocrinology, vol. 92, no. 5, pp. 1555-1559.
Fleischer N, McMillin Wood J. Ouabain stimulation of 45Ca++ accumulation in the rat pituitary. Endocrinology. 1973;92(5):1555-1559.
Fleischer, N. ; McMillin Wood, J. / Ouabain stimulation of 45Ca++ accumulation in the rat pituitary. In: Endocrinology. 1973 ; Vol. 92, No. 5. pp. 1555-1559.
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