Ouabain stimulation of 45ca++ accumulation in the rat pituitary

Norman Fleischer; Jeanie Mcmillin Wood

doi:10.1210/endo-92-5-1555

Ouabain stimulation of ⁴⁵ca⁺⁺ accumulation in the rat pituitary

Norman Fleischer, Jeanie Mcmillin Wood

Research output: Contribution to journal › Article › peer-review

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Abstract

Anterior pituitary tissue from rats was incubated at 37 C in Krebs’ Ringer’s bicarbonate in an atmosphere of 95% O₂ and 5% CO₂ with hourly changes of the incubation media. After a one hour preincubation, ⁴⁵Ca⁺⁺ was added to the media and its uptake by the pituitary tissue was determined. The digitalis glycoside, ouabain, in concentrations of 5 X 10^-4 and 1 × 10^-3M significantly augmented pituitary ⁴⁵Ca⁺⁺. This effect was dose and time dependent. Ouabain did not alter the washout kinetics for ¹⁴C inulin or affect pituitary weights suggesting that the observed changes in ⁴⁵Ca⁺⁺ content were the result of increased ⁴⁵Ca⁺⁺ accumulation. The effect of ouabain on calcium flux may be related to the stimulation of ACTH and GH release by ouabain.

Original language	English (US)
Pages (from-to)	1555-1559
Number of pages	5
Journal	Endocrinology
Volume	92
Issue number	5
DOIs	https://doi.org/10.1210/endo-92-5-1555
State	Published - May 1973
Externally published	Yes

ASJC Scopus subject areas

Endocrinology

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title = "Ouabain stimulation of 45ca++ accumulation in the rat pituitary",

abstract = "Anterior pituitary tissue from rats was incubated at 37 C in Krebs{\textquoteright} Ringer{\textquoteright}s bicarbonate in an atmosphere of 95% O2 and 5% CO2 with hourly changes of the incubation media. After a one hour preincubation, 45Ca++ was added to the media and its uptake by the pituitary tissue was determined. The digitalis glycoside, ouabain, in concentrations of 5 X 10-4 and 1 × 10-3M significantly augmented pituitary 45Ca++. This effect was dose and time dependent. Ouabain did not alter the washout kinetics for 14C inulin or affect pituitary weights suggesting that the observed changes in 45Ca++ content were the result of increased 45Ca++ accumulation. The effect of ouabain on calcium flux may be related to the stimulation of ACTH and GH release by ouabain.",

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AU - Wood, Jeanie Mcmillin

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AB - Anterior pituitary tissue from rats was incubated at 37 C in Krebs’ Ringer’s bicarbonate in an atmosphere of 95% O2 and 5% CO2 with hourly changes of the incubation media. After a one hour preincubation, 45Ca++ was added to the media and its uptake by the pituitary tissue was determined. The digitalis glycoside, ouabain, in concentrations of 5 X 10-4 and 1 × 10-3M significantly augmented pituitary 45Ca++. This effect was dose and time dependent. Ouabain did not alter the washout kinetics for 14C inulin or affect pituitary weights suggesting that the observed changes in 45Ca++ content were the result of increased 45Ca++ accumulation. The effect of ouabain on calcium flux may be related to the stimulation of ACTH and GH release by ouabain.

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Ouabain stimulation of ⁴⁵ca⁺⁺ accumulation in the rat pituitary

Abstract

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