Oscillating Couette flow for in vitro cell loading

Razi Nalim, Kerem Pekkan, Hui (Herb) Sun, Hiroki Yokota

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Synovial joints are loaded by weight bearing, stretching, and fluid-driven shear. To simulate in vitro fluid-driven shear, we developed an "oscillating Couette flow mechanical shear loader". Oscillating Couette flow mimics relative motion of articular surfaces; hence, characterizing flow-induced shear by the loader enhances understanding of mechanotransduction in the joint tissue. Here, the analytical and computational models for an oscillating Couette flow were used to predict time-varying shear distribution on a plate surface, applying numerical simulation to evaluate the effects of finite plate dimension in a 2D flow. Shear stress on the plate was significantly different from that in simpler models (unbounded plates and viscous low-frequency flow). High-stress spots appeared near the leading and trailing edges of a moving plate, and a relatively uniform shear region was restricted to the interior area. Stress prediction in an example experimental geometry is presented, where the frequency and finite width effects are feasibly accounted.

Original languageEnglish (US)
Pages (from-to)939-942
Number of pages4
JournalJournal of Biomechanics
Volume37
Issue number6
DOIs
StatePublished - Jun 2004
Externally publishedYes

Fingerprint

Oscillating flow
Loaders
Joints
Bearings (structural)
Fluids
Weight-Bearing
Stretching
Shear stress
Tissue
Geometry
Computer simulation
In Vitro Techniques

Keywords

  • Articular surface
  • Computational fluid dynamics
  • Mechanotransduction
  • Oscillating flow
  • Shear stress
  • Synovial joint

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Oscillating Couette flow for in vitro cell loading. / Nalim, Razi; Pekkan, Kerem; Sun, Hui (Herb); Yokota, Hiroki.

In: Journal of Biomechanics, Vol. 37, No. 6, 06.2004, p. 939-942.

Research output: Contribution to journalArticle

Nalim, Razi ; Pekkan, Kerem ; Sun, Hui (Herb) ; Yokota, Hiroki. / Oscillating Couette flow for in vitro cell loading. In: Journal of Biomechanics. 2004 ; Vol. 37, No. 6. pp. 939-942.
@article{39861f5960214faba7e6884caaecaa8c,
title = "Oscillating Couette flow for in vitro cell loading",
abstract = "Synovial joints are loaded by weight bearing, stretching, and fluid-driven shear. To simulate in vitro fluid-driven shear, we developed an {"}oscillating Couette flow mechanical shear loader{"}. Oscillating Couette flow mimics relative motion of articular surfaces; hence, characterizing flow-induced shear by the loader enhances understanding of mechanotransduction in the joint tissue. Here, the analytical and computational models for an oscillating Couette flow were used to predict time-varying shear distribution on a plate surface, applying numerical simulation to evaluate the effects of finite plate dimension in a 2D flow. Shear stress on the plate was significantly different from that in simpler models (unbounded plates and viscous low-frequency flow). High-stress spots appeared near the leading and trailing edges of a moving plate, and a relatively uniform shear region was restricted to the interior area. Stress prediction in an example experimental geometry is presented, where the frequency and finite width effects are feasibly accounted.",
keywords = "Articular surface, Computational fluid dynamics, Mechanotransduction, Oscillating flow, Shear stress, Synovial joint",
author = "Razi Nalim and Kerem Pekkan and Sun, {Hui (Herb)} and Hiroki Yokota",
year = "2004",
month = "6",
doi = "10.1016/j.jbiomech.2003.11.004",
language = "English (US)",
volume = "37",
pages = "939--942",
journal = "Journal of Biomechanics",
issn = "0021-9290",
publisher = "Elsevier Limited",
number = "6",

}

TY - JOUR

T1 - Oscillating Couette flow for in vitro cell loading

AU - Nalim, Razi

AU - Pekkan, Kerem

AU - Sun, Hui (Herb)

AU - Yokota, Hiroki

PY - 2004/6

Y1 - 2004/6

N2 - Synovial joints are loaded by weight bearing, stretching, and fluid-driven shear. To simulate in vitro fluid-driven shear, we developed an "oscillating Couette flow mechanical shear loader". Oscillating Couette flow mimics relative motion of articular surfaces; hence, characterizing flow-induced shear by the loader enhances understanding of mechanotransduction in the joint tissue. Here, the analytical and computational models for an oscillating Couette flow were used to predict time-varying shear distribution on a plate surface, applying numerical simulation to evaluate the effects of finite plate dimension in a 2D flow. Shear stress on the plate was significantly different from that in simpler models (unbounded plates and viscous low-frequency flow). High-stress spots appeared near the leading and trailing edges of a moving plate, and a relatively uniform shear region was restricted to the interior area. Stress prediction in an example experimental geometry is presented, where the frequency and finite width effects are feasibly accounted.

AB - Synovial joints are loaded by weight bearing, stretching, and fluid-driven shear. To simulate in vitro fluid-driven shear, we developed an "oscillating Couette flow mechanical shear loader". Oscillating Couette flow mimics relative motion of articular surfaces; hence, characterizing flow-induced shear by the loader enhances understanding of mechanotransduction in the joint tissue. Here, the analytical and computational models for an oscillating Couette flow were used to predict time-varying shear distribution on a plate surface, applying numerical simulation to evaluate the effects of finite plate dimension in a 2D flow. Shear stress on the plate was significantly different from that in simpler models (unbounded plates and viscous low-frequency flow). High-stress spots appeared near the leading and trailing edges of a moving plate, and a relatively uniform shear region was restricted to the interior area. Stress prediction in an example experimental geometry is presented, where the frequency and finite width effects are feasibly accounted.

KW - Articular surface

KW - Computational fluid dynamics

KW - Mechanotransduction

KW - Oscillating flow

KW - Shear stress

KW - Synovial joint

UR - http://www.scopus.com/inward/record.url?scp=1942509655&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1942509655&partnerID=8YFLogxK

U2 - 10.1016/j.jbiomech.2003.11.004

DO - 10.1016/j.jbiomech.2003.11.004

M3 - Article

C2 - 15111082

AN - SCOPUS:1942509655

VL - 37

SP - 939

EP - 942

JO - Journal of Biomechanics

JF - Journal of Biomechanics

SN - 0021-9290

IS - 6

ER -